FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5695423
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGC1468642.5786319997654257RNA PCR Primer, Index 33 (100% over 50bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC340810.5983927796056587RNA PCR Primer, Index 2 (100% over 34bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT309110.5427340515357683RNA PCR Primer, Index 1 (100% over 25bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG268960.47223884863336757RNA PCR Primer, Index 7 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA222540.39073480582566034No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATGTCGTATGC209800.3683659668474142RNA PCR Primer, Index 33 (98% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA191820.33679675767717343RNA PCR Primer, Index 7 (100% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA175830.30872158222488477No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG169610.2978005321114867No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA156910.2755019249667672No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC155670.2733247381274402No Hit
CTTCGGTCGAGAGCATGCCTGTCTCAGCGTCGGCTGGAATTCTCGGGTGC144310.25337889740586433No Hit
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT132380.23243225305653328RNA PCR Primer, Index 1 (100% over 25bp)
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT102670.1802675587045949RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA91120.15998811677376729No Hit
CCGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTGGAATTCTCGG78960.13863763938165788No Hit
GTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTGGAATTCTCGGGT77330.13577569216544583No Hit
AGATAGTACTGATCTGCTGCGACGAGTGACTGTCTGGAATTCTCGGGTGC73730.12945482714804502No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGG70420.12364314292371259RNA PCR Primer, Index 31 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGAGCTCGTATGC69400.12185223116878237RNA PCR Primer, Index 33 (98% over 50bp)
GGTGCTGGTCAGCATCGGTTGGCGGGCCGGGAGAAAAGCGGTGGAATTCT57360.100712449277253No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)