FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002798724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817469
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC424625.194325411728151RNA PCR Primer, Index 18 (100% over 50bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG70140.8580141876939676RNA PCR Primer, Index 6 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA66200.8098166413649935No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTGGAATTCTCGGGTGCCA60940.7454716937278356No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA59860.732260183566594No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT45870.5611221954593997RNA PCR Primer, Index 1 (100% over 25bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC44750.547421370107001RNA PCR Primer, Index 1 (97% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT34650.4238692843398343RNA PCR Primer, Index 1 (97% over 35bp)
AGCATTGGCGTGTAGAGGGGGATACGCGGAGGGTTTCGACTGGAATTCTC25070.30667829605770985No Hit
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT18610.22765389268583885RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCGCGTATGC18480.22606361831457827RNA PCR Primer, Index 18 (98% over 50bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT18180.2223937543809001RNA PCR Primer, Index 1 (100% over 25bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC16670.2039221059147197RNA PCR Primer, Index 18 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCAGCTCGTATGC15300.18716306061758917RNA PCR Primer, Index 18 (98% over 50bp)
AGATAGTACTGATCTGCTGCGACGAGTGACTGTCTGGAATTCTCGGGTGC14380.17590881122097599No Hit
CGGGCTGTGGCGCAGTTTGGTAGCGCACTTGACTTGGAATTCTCGGGTGC12550.15352264122553883No Hit
CAACTCTCAACAACGGATATCTTGGCTCTTGGAATTCTCGGGTGCCAAGG9670.11829194746222793Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGAGTGGCGGAATGGTAGACGCGGCGCACTCTGGAATTCTCGGGTGC9500.11621235789981027No Hit
TTCGACCTGAGCTCAGGCAAGGCTACCCTGGAATTCTCGGGTGCCAAGGA9190.11242016516834277RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)