FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5918076
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGTATGC5601119.464410392837133RNA PCR Primer, Index 36 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1199292.0264863107536977No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC459420.7762995946655635RNA PCR Primer, Index 2 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCA334320.564913326560862RNA PCR Primer, Index 2 (97% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC300170.507208761766493RNA PCR Primer, Index 5 (97% over 35bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT299520.5061104318362928RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATGTCGTATGC267190.4514811908464845RNA PCR Primer, Index 36 (98% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA226950.38348611947531597No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC166730.28173007578814463No Hit
TATGCATATGATCACGAGACTCAGTTGGAATTCTCGGGTGCCAAGGAACT133880.22622217085417626RNA PCR Primer, Index 1 (100% over 25bp)
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT118730.20062263478873876RNA PCR Primer, Index 1 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA111790.188895850610908No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAAGCTCGTATGC101770.17196467230228202RNA PCR Primer, Index 36 (98% over 50bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAA95870.1619952160127717RNA PCR Primer, Index 28 (97% over 37bp)
AGATAGTACTGATCTGCTGCGACGAGTGACTGTCTGGAATTCTCGGGTGC83350.14083969181876002No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA75000.1267303765615717RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCGCGTATGC62780.10608177387380628RNA PCR Primer, Index 36 (98% over 50bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGT61250.10349647419195021RNA PCR Primer, Index 36 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)