FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11235797
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGTCGTTGGTTCAAGCCCATCCTGAAGCGCCATGGAATTCTCGGGT3596853.2012415318646283No Hit
GGAGAGGTGTCCGAGCGGCTGAGGGAGCATGATTGGAATTCTCGGGTGCC2471902.2000219477087386No Hit
TGCCCGTGACTATACAGACGAGGAAACGCCTTGGAATTCTCGGGTGCCAA1291701.14962917183356No Hit
AGGAGAAAGATCAATGTAGTGACGAAGACTATGGAATTCTCGGGTGCCAA1078400.9597895013589156No Hit
AGGGGGTCGTTGGTTCAAGCCCATCCTGGAGCGCCATGGAATTCTCGGGT1007380.8965808122022852No Hit
GCGGCAATAGCTCAGTTGGTAGAGCACGTGGAATTCTCGGGTGCCAAGGA974000.866872194291157RNA PCR Primer, Index 1 (100% over 22bp)
GGTGGATGTAGTTCAGTTGGTTAGAGCGTCTGGAATTCTCGGGTGCCAAG864220.7691666198668416No Hit
AGGGGGTCGTTGGTTCAAGCCCATCCTGGAGCGCCTGGAATTCTCGGGTG706620.6289006467453978No Hit
GGAGAAAGATCAATGTAGTGACGAAGACTATGGAATTCTCGGGTGCCAAG662690.5898023967503151No Hit
TAGGAGAAAATCAATGTAGCGACTAAGACTATGGAATTCTCGGGTGCCAA661530.58876998222734No Hit
GGCCCATTCGTCTAGCGGTTAGGACATCTGGAATTCTCGGGTGCCAAGGA589280.5244665776713481RNA PCR Primer, Index 1 (100% over 22bp)
GGGTCTGTAGCTCAGCTGGTTAGAGCACCTGGAATTCTCGGGTGCCAAGG565600.5033910812023393Illumina Small RNA Adapter 2 (100% over 21bp)
GGAGAGATGGTAGAGTGGTCGATTACAACTGGAATTCTCGGGTGCCAAGG556950.49569247290601637Illumina Small RNA Adapter 2 (100% over 21bp)
AGGAGAAAGACCAATGTAGCGACTAAGACTATGGAATTCTCGGGTGCCAA486780.43324029439122125No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACCTGGAATTCTCGGGTGCCAAGGA467590.41616095413614185RNA PCR Primer, Index 1 (100% over 22bp)
AGGGGGTCGTTGGTTCAAGCCCATCCTGAAGCGCCTGGAATTCTCGGGTG464970.41382912133424976No Hit
GCGGAAGTAGCTCAGTGGTAGAGCACCTGGAATTCTCGGGTGCCAAGGAA410890.36569724426313505RNA PCR Primer, Index 1 (100% over 23bp)
GGTGAGTGTAGCTCAGTCGGTTAGAGCATCTGGAATTCTCGGGTGCCAAG378740.33708334175136845No Hit
GGTTGGATAGCTCAGTCGGTAGAGCAGCTGGAATTCTCGGGTGCCAAGGA374230.33306938528704283RNA PCR Primer, Index 1 (100% over 22bp)
AGGAGAAAATCAATGTAGCGACTAAGACTATGGAATTCTCGGGTGCCAAG355620.31650625229345103No Hit
CGCGGGTTGGAGCAGTTGGTAGCTCGCCTGGAATTCTCGGGTGCCAAGGA340330.3028979608656155RNA PCR Primer, Index 1 (100% over 22bp)
GGAAGGATGGGTGAGTGGCTGAAACCAGCTGGAATTCTCGGGTGCCAAGG329790.29351722890685905Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGAATTAGCACATCGGTAGTGCACGTGGAATTCTCGGGTGCCAAGGAA329480.29324132502571915RNA PCR Primer, Index 1 (100% over 23bp)
GGAGAAAGACCAATGTAGCGACTAAGACTATGGAATTCTCGGGTGCCAAG302630.2693444888689249No Hit
GCCCAGATGGCGGAATAGGTAGACGCGCTTGGAATTCTCGGGTGCCAAGG275870.24552775383891323Illumina Small RNA Adapter 2 (100% over 21bp)
GGAGAGATGGTAGAGTGGTCGATTACAACGGTCTTTGGAATTCTCGGGTG262190.2333523825679656No Hit
ATGGCGTGGGCTTTTACTCGTGTATGATGTGCTTGGAATTCTCGGGTGCC253900.22597417877877288No Hit
GGAGAGGTGGCGGAATTGGTAGACGCGCTTGGAATTCTCGGGTGCCAAGG252270.2245234583714889Illumina Small RNA Adapter 2 (100% over 21bp)
GATCGGTCGTAGGTTCGAGTCCTACTCGGGGAGCCATGGAATTCTCGGGT248000.2207231049119168No Hit
GGAGAGATGGTAGAGTGGTCGATTACAACGTGGAATTCTCGGGTGCCAAG240450.2140035103873806No Hit
TCCGGATTAGCTCAGCGGTAGAGTAGGTGGAATTCTCGGGTGCCAAGGAA229990.20469397943020864RNA PCR Primer, Index 1 (100% over 23bp)
GGAAGGATGGGTGAGTGGCTGAAACCAGCAGTTTGCTGGAATTCTCGGGT225710.20088472584543848No Hit
GGCCCATTCGTCTAGCGGTTAGGACACCTGGAATTCTCGGGTGCCAAGGA224730.20001251357602848RNA PCR Primer, Index 1 (100% over 22bp)
GGTGGACGTAGTTCAGTTGGTTAGAGCGTCTGGAATTCTCGGGTGCCAAG219330.19520644596907546No Hit
TAGGAGAAAGACCAATGTAGCGACTAAGACTATGGAATTCTCGGGTGCCA208300.18538960787561398No Hit
GGGTCTGTAGCTCAGGTGGTTAGAGCGCACCTGGAATTCTCGGGTGCCAA208050.18516710474566245No Hit
CGCGGAGTAGAGCAGTGGTAGCTCGTCGTGGAATTCTCGGGTGCCAAGGA206690.18395668771872614RNA PCR Primer, Index 1 (100% over 22bp)
GCAGCGGTAGTTCAGTTGGTTAGAATGCCTGGAATTCTCGGGTGCCAAGG203130.18078824314821637Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGAGTGGAGCAGTTGGTAGCTCGCCTGGAATTCTCGGGTGCCAAGGA199380.1774506961989434RNA PCR Primer, Index 1 (100% over 22bp)
GGAGAGATGGTAGAGTGGTCGATTACAACGGTCTTGGAATTCTCGGGTGC198790.17692558881225784No Hit
GCGGAAATAGCTCAGTTGATAGAGCACTTGGAATTCTCGGGTGCCAAGGA197820.1760622766680459RNA PCR Primer, Index 1 (100% over 22bp)
GGTGGATGTAGTTCAGTTGGTTAGAGCGTCATGGAATTCTCGGGTGCCAA197750.17599997579165946No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC189920.16903117776157758No Hit
AAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTGCCTGGAATTCTCGGGTGC186360.16586273319106778No Hit
AGTAGGTGATTGGTTCGACTCCGATAAGCGGCACCATGGAATTCTCGGGT182910.1627921899977367No Hit
TTCAGATTGCACATACACTGAACCGGTTGTGATTTGCTTGGAATTCTCGG180220.16039805631945825No Hit
CGCGGAGTGGAGCAGTTGGTAGCTCGCCAGGCTCTGGAATTCTCGGGTGC176800.15735421350172135No Hit
GTCCCCATCGTCTAGAGGCCTAGGACATCGCCCTGGAATTCTCGGGTGCC167090.14871219193440394No Hit
GGAGAGGTGTCCGAGCGGCTGATGGAGCATGATTGGAATTCTCGGGTGCC162260.1444134314637404No Hit
GGGGACATAGCTCAGTTGGCTAGAGCACCTGGAATTCTCGGGTGCCAAGG160890.14319411431160603Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGAAGTAGCTCAGTGGTAGAGCACCACCTTGGAATTCTCGGGTGCCAA156380.13918015784728044No Hit
GCGGAAGTAGCTCAGTGGTAGAGCACCACTGGAATTCTCGGGTGCCAAGG154470.13748023393445077Illumina Small RNA Adapter 2 (100% over 21bp)
AGCACTAATCGGTCGAGGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC150540.13398248473161273RNA PCR Primer, Index 1 (100% over 24bp)
GGAGGTCGGCAGTTCAAGTCTGCCTGGGACTTGGAATTCTCGGGTGCCAA150040.13353747847170966No Hit
GGAGAGGTGTCCGAGCGGCTGAAGGAGCATGATTGGAATTCTCGGGTGCC148490.1321579590660102No Hit
TCCGGATTAGCTCAGCGGTAGAGTAGGTTGGAATTCTCGGGTGCCAAGGA147180.13099204266506417RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGAGTAGAGCAGTGGTAGCTCGTCTGGAATTCTCGGGTGCCAAGGAA145370.1293811200042151RNA PCR Primer, Index 1 (100% over 23bp)
GGTGCGTTAGTTCAGTTGGTTAGAATGCCTTGGAATTCTCGGGTGCCAAG145220.1292476181262442No Hit
GGTGACCGTAGTTCAGTTGGTAGAGCGCCTGGAATTCTCGGGTGCCAAGG144040.12819740335287297Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCAGGTGGCGGAATTGGTAGACGCGCATGGAATTCTCGGGTGCCAAGG143700.12789479909613888Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGCGTTAGTTCAGCTGGTTAGAATGCCTTGGAATTCTCGGGTGCCAAG142780.12707598757791724No Hit
GGTGGCTGTAGCTCAGTTGGTAGAGTCCCTGGAATTCTCGGGTGCCAAGG138520.1232845342435432Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGCCGCAGGGGTTGTAGGACCACGCTGGAATTCTCGGGTGCCAAGGAA137150.12206521709140883RNA PCR Primer, Index 1 (100% over 23bp)
TGTCCGTGATTATACAGATGTGGAAACGCCTTGGAATTCTCGGGTGCCAA136180.1212019049471969No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA134830.12000038804545865No Hit
TTACATAACAGATTGTAGAGAAGTACAAGGAACAATTGGAATTCTCGGGT132330.11777535674594335No Hit
GCGGAATTAGCACATCGGTAGTGCACGGGCTTCCTGGAATTCTCGGGTGC130730.11635133671425357No Hit
GGAAGGATGGGTGAGTGGCTGAAACCAGCATGGAATTCTCGGGTGCCAAG128430.11430430791869949No Hit
CGGGGTGTAGCGCAGTCTGGTTAGCGCATCTGGAATTCTCGGGTGCCAAG121990.10857262729114811No Hit
GCGGAAGTAGCTCAGTGGTAGAGCACCACCTTGCCTGGAATTCTCGGGTG120690.10741561101540015No Hit
AGGGGGTCGTTGGTTCAAGCCCATCTTGAAGCGCCATGGAATTCTCGGGT115850.10310795041953856No Hit
GGGGACGTAGCTCAGCTGGGAGAGCACCTGGAATTCTCGGGTGCCAAGGA114510.10191533364299836RNA PCR Primer, Index 1 (100% over 22bp)
TCTAAAGAGCAGTTCGACTCTGCTAGGGACTACCTGGAATTCTCGGGTGC112770.10036671185853573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)