FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11291727
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTCAATGGTAGAACACCTGGAATTCTCGGGTGCCAAGGAA930760.8242848945958399RNA PCR Primer, Index 1 (100% over 23bp)
ATCGGAGAAGAACTTTCTGGAAAGAAATGGAATTCTCGGGTGCCAAGGAA908340.8044296501323491RNA PCR Primer, Index 1 (100% over 23bp)
AGGATAGGAGAACGGTCTGGGAAGTCCTGGAATTCTCGGGTGCCAAGGAA874960.7748681844681509RNA PCR Primer, Index 1 (100% over 23bp)
ACCGAAGAAGGTGAGAGTCCTGTACACGAAAAGCCTTGGAATTCTCGGGT778370.6893276821162962No Hit
CGCGGGATGGAGCAGTCTGGAAGCTCGCCGGGCTCTGGAATTCTCGGGTG683140.6049916013732887No Hit
GTTCGGCCGGGCACTCTGGAGAGACTGCCTGGAATTCTCGGGTGCCAAGG505770.4479119978724247Illumina Small RNA Adapter 2 (100% over 21bp)
ACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGTGGAATTCTCGGG480320.42537337291275285No Hit
TCGGAGAAGAACTTTCTGGAAAGAAATGGAATTCTCGGGTGCCAAGGAAC458410.4059697865525796RNA PCR Primer, Index 1 (100% over 24bp)
ATTGTTTCGGCAGTAGCGGGGTAGTAGGACTGCTGGAATTCTCGGGTGCC437110.38710641870813917No Hit
GGCAACATGTGGAGCTGACTGTTACTAATAGGTCGAGGGCTTAATTGGAA429830.3806592206843116No Hit
CGCGGGATGGAGCAGTCTGGAAGCTCGCCGGGCTTGGAATTCTCGGGTGC405530.3591390404674148No Hit
TGGTGAGTAGAATGTTCTGGAAAGTTCTGGAATTCTCGGGTGCCAAGGAA386470.3422594258610751RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGAGTGGAGCAGTTGGTAGCTCGTTGGGCTCTGGAATTCTCGGGTGC384520.34053249781897843No Hit
GTGATGCTGAGGACTCTGACAAGACTGCCTGGAATTCTCGGGTGCCAAGG382650.33887641810681396Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGCTAAATCGAAAGATGACGTATACGAGCTGACGCCTGGAATTCTCGG371630.3291170606586574No Hit
AACCGAAGAAGGTGAGAGTCCTGTACACGAAAAGCCTTGGAATTCTCGGG360420.319189438426912No Hit
TTACCGAAGCCTATCAAACTCTGAATGCCGAATATGGAATTCTCGGGTGC325180.28798074909179083No Hit
TCAGTTACTACGCAAGAGGACTCTGGCCAGACTGCCTGGAATTCTCGGGT298350.2642199904407891No Hit
CTGGTGAGTAGAATGTTCTGGAAAGTTCTGGAATTCTCGGGTGCCAAGGA296210.2623247976151035RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGGAAACACGGGTTCGATTCCCGTACGGACTGCTGGAATTCTCGGGT294080.26043846083065947No Hit
GTGTCTGTAGCTCAGTTGGATAGAGCACCTGGAATTCTCGGGTGCCAAGG293840.26022591584086296Illumina Small RNA Adapter 2 (100% over 21bp)
AATCTGTTAACCGAACGGCATGGGAAGGCCTGGAATTCTCGGGTGCCAAG260120.230363344774453No Hit
ACAGGATAGGAGAACGGTCTGGGAAGTCCTGGAATTCTCGGGTGCCAAGG254290.22520027273064605Illumina Small RNA Adapter 2 (100% over 21bp)
AACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGTGGAATTCTCGG229930.20362695626630012No Hit
ATCGGAGAAGAACTTTCTGGAAAGAAAGTGGAATTCTCGGGTGCCAAGGA229810.20352068377140184RNA PCR Primer, Index 1 (100% over 22bp)
TAGAGGTCGGCAGTTCAACTCTGCCTGGGACTACCTGGAATTCTCGGGTG223200.19766684051075625No Hit
AGTGGCGGACGGGTGAGTAACACGTGAGCAACCTGGCTCTTGGAATTCTC220760.19550596644782506No Hit
GGCAAGAGATTAAGGAAGTAGAACGTTTTGGAACAAACTGGAATTCTCGG220390.19517829292188874No Hit
GGTGGATTCGTCTAACGGTTAGGACACATGCCTTGGAATTCTCGGGTGCC217620.19272516949798735No Hit
ACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGAGTGGAATTCTCG196510.17403006643713578No Hit
TGGCGGACGGGTGAGTAACACGTGAGCAACCTGGCTCTTGGAATTCTCGG186070.1647843593809875No Hit
CGGGGTGTAGCGCAGTCCGGTTAGCGCACCTGGAATTCTCGGGTGCCAAG183970.16292459072026802No Hit
GGTGATGCTGAGGACTCTGACAAGACTGCCTGGAATTCTCGGGTGCCAAG179690.1591342050688969No Hit
TCAGTTACTACGCAAGAGGACTCTGGCAGGACTGCCTGGAATTCTCGGGT178130.15775266263521956No Hit
ACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGATGGAATTCTCGG176820.15659252123258027No Hit
TTGTTTCGGCAGTAGCGGGGTAGTAGGACTGCTGGAATTCTCGGGTGCCA175300.15524640296386902No Hit
TTACCGAAGAATATCAAACTCCGAATGCCATATTGGAATTCTCGGGTGCC173740.1538648605301917No Hit
GTTCGGCCGGGCACTCTGGAGAGACTGCCATGGAATTCTCGGGTGCCAAG167130.1480110172695461No Hit
GCCGGAGAGGGTGAAAGCCCCGTAAGCGAAAGGAGAGCTGGAATTCTCGG166660.1475947833311946No Hit
TCTTCCTTAGCTCAGTCGGTTAGAGCATCTGACTTGGAATTCTCGGGTGC160430.1420774696377268No Hit
TAGTGAGAAGAACCCTTTGGAAAATGGTGGAATTCTCGGGTGCCAAGGAA157710.139668626420033RNA PCR Primer, Index 1 (100% over 23bp)
TGGCAACATGTGGAGCTGACTGTTACTAATAGGTCGAGGGCTTAATTGGA157420.13941180122402888No Hit
GTGGCGGACGGGTGAGTAACACGTGAGCAACCTGGCTCTTGGAATTCTCG156360.1384730608524276No Hit
GGCAACATGTGGAGCTGACTGTTACTAATAGGTCGAGGGCTGGAATTCTC155800.13797712254290243No Hit
CATCGAAGAGTGTGAGAATCACGTAGGCGAAACGGGAATGGAATTCTCGG155800.13797712254290243No Hit
AACCGAAGAAGGTGAGAGTCCTGTACACGAAAAGCCTGAGCATGGAATTC153910.1363033307482549No Hit
CATCGGAGAAGAACTTTCTGGAAAGAAATGGAATTCTCGGGTGCCAAGGA152100.13470038728353953RNA PCR Primer, Index 1 (100% over 22bp)
TTGAAAGGGGGGTTCGATTCCCCCACGGGCTACCATGGAATTCTCGGGTG149470.13237124843701942No Hit
GTTCGGCCGGGCACTCTAGAGAGACTGCCTGGAATTCTCGGGTGCCAAGG149360.13227383198336268Illumina Small RNA Adapter 2 (100% over 21bp)
TGATTGTTAACCGAACAGCATGGGAAGGCTTGGAATTCTCGGGTGCCAAG147120.1302900787452619No Hit
ACTGGACGATAACTGACGCTGAGGCTCGAAAGCGTTGGAATTCTCGGGTG144340.12782809928011896No Hit
TACTGGACGATAACTGACGCTGAGGCTCGAAAGCGTTGGAATTCTCGGGT143980.1275092817954242No Hit
AGAGATGGGCTCGCGTCTGATTAGATAGTTGGCGGTGGAATTCTCGGGTG140030.12401114550502329No Hit
AACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGAGTGGAATTCTC138930.12303698096845592No Hit
CGCGGGATGGAGCAGTCTGGAAGCTCGCCTGGAATTCTCGGGTGCCAAGG135860.12031817630730889Illumina Small RNA Adapter 2 (100% over 21bp)
CAAGATTATCGGAGGTAAAGCACTGAATGGCCTGGAATTCTCGGGTGCCA135240.11976910175033456No Hit
ACCGAAGAAGGTGAGAGTCCTGTACACGAAAAGCCTGTGGAATTCTCGGG134740.1193262996882585No Hit
ACCGAAGAAGGTGAGAGTCCTGTACACGAAAAGCCTGAGCATGGAATTCT134100.11875951304880113No Hit
TCTTTAGTCGAACAGTCTGGGAAGGCTTGGAATTCTCGGGTGCCAAGGAA133150.11791818913085661RNA PCR Primer, Index 1 (100% over 23bp)
TGTGCTAAATCGAAAGATCCTGTATACAGCCTGACACTGGAATTCTCGGG131510.1164657983672471No Hit
ACCGAAGAAGGTGAGAGTCCTGTACACGAAAAGCCTGGAATTCTCGGGTG130000.11512853613977739No Hit
GTTCCGTTAGCTCAGCTGGTAGAGCATCTGACTGGAATTCTCGGGTGCCA128950.11419865180941763No Hit
CAGGATAGGAGAACGGTCTGGGAAGTCCTGGAATTCTCGGGTGCCAAGGA128480.11378241787106615RNA PCR Primer, Index 1 (100% over 22bp)
AGGAAGCTAGGAGAACGGCATGGGAAGGCCTGGAATTCTCGGGTGCCAAG127980.11333961580899006No Hit
AACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGATGGAATTCTCG127970.11333075976774855No Hit
CGTGAGGCATTCAGCTTGGCAGTACTAATAGGTCGAGGGCTTGACTGGAA125280.11094848467377931No Hit
TATGCAAAACCGAAAGGTGAGGTATATGGGCTGGAATTCTCGGGTGCCAA124790.11051453865294476No Hit
AACCAAAGAGTGTGAGAGTCACGTAGATGAAAACGGCGAGATGGAATTCT122530.10851307333236095No Hit
CGGGGTGTGGCTCAGCTTGGCTAGAGCGCCTGGAATTCTCGGGTGCCAAG121000.10715809902240817No Hit
CGAAGAACTCGTATCATCCATACTGGAATTCTCGGGTGCCAAGGAACTCC120620.10682156945523036RNA PCR Primer, Index 1 (100% over 27bp)
GGCAAGAAAGTTGGTGAGCAGAATGTTCTGGAAAGTACTGGAATTCTCGG120560.10676843320778125No Hit
GTTCCGTTAGCTCAGCTGGTAGAGCATCTGTGGAATTCTCGGGTGCCAAG120370.10660016842419233No Hit
GGGGACGTAGCTCAGTTGGGAGAGCACCTGGAATTCTCGGGTGCCAAGGA119590.10590939720735365RNA PCR Primer, Index 1 (100% over 22bp)
AGAAGATAGTAGAAGGGCCTGGGAAGTCCTGGAATTCTCGGGTGCCAAGG118870.10527176223796413Illumina Small RNA Adapter 2 (100% over 21bp)
GGCAAGAAAACTGGTGAGTAGAATGTTCTGGAAAGTTCTGGAATTCTCGG118140.10462527122733307No Hit
TTAGCGAAGCTTATCAAACTCAGAATGCCAGAATGGAATTCTCGGGTGCC117100.10370424293821486No Hit
CGGGGTGTGGCTCAGCTTGGCTAGAGCGCCTTGGAATTCTCGGGTGCCAA116480.10315516838124053No Hit
GCGGATTTAGCTCATCTGGTAGAGCGCCTGGAATTCTCGGGTGCCAAGGA116020.10274779048413056RNA PCR Primer, Index 1 (100% over 22bp)
GAGGAGCAATCCTTTCGGGGTTTAGGACTGCTGGAATTCTCGGGTGCCAA115110.1019418907311521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)