FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6217293
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC67989510.93554702987297RNA PCR Primer, Index 32 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG3523955.66798122591295No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG1645782.647100595066052No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1410832.269203011664401No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1273612.0484960255210747No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1106741.7800994741602172No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT1021121.6423868072487497No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG618840.9953528006481277Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA613020.9859918134789529RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC411220.6614132549326531No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA384110.6178090689951399RNA PCR Primer, Index 1 (100% over 22bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC343370.5522821588109166RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA273500.43990206026963824No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG267590.43039631556691954No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG256800.41304149571204063Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA251490.4045008012329481RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA237230.38156477425143065No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA219070.3523559208163424RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA209530.3370116222606848RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAGCTCGTATGC198190.31877217303414845RNA PCR Primer, Index 32 (98% over 50bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC191250.3076097587808713RNA PCR Primer, Index 2 (100% over 34bp)
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG155230.24967457702250803No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA146410.23548833873520197No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAA144780.23286661896101726No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATGTCGTATGC143160.2302609833572264RNA PCR Primer, Index 32 (98% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC142620.22939243815596275No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC138200.22228323484191592RNA PCR Primer, Index 1 (100% over 24bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT130690.21020402287619386RNA PCR Primer, Index 1 (100% over 25bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC129490.20827392242894135RNA PCR Primer, Index 30 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATATCGTATGC118200.19011489405437382RNA PCR Primer, Index 32 (98% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC107410.17276007419949488No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA102630.1650718407512723RNA PCR Primer, Index 7 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCGCGTATGC98060.15772137488131893RNA PCR Primer, Index 32 (98% over 50bp)
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT93800.15086951829357245No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTGGAATTCTCGGGTGCC86370.1389189796910006No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC82230.13226013314797935No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG72580.11673890871799028No Hit
CGATGCTATAGACTCTCACGTGATGTGGAATTCTCGGGTGCCAAGGAACT65680.10564083114628826RNA PCR Primer, Index 1 (100% over 25bp)
AAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTC65560.105447821101563No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA64580.10387157240297346RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA63120.10152328352548287RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)