FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002798731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5900020
Sequences flagged as poor quality0
Sequence length76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA114087719.33683275649913No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG4019326.812383686834959No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1461802.4776187199365425No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1329172.2528228717868752No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1172041.9865017406720655Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG732741.241927993464429Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA697761.1826400588472583No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA498840.8454886593604768RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG487670.8265565201473893No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA442160.7494211884027512RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC436100.7391500367795363No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA314300.5327100586099708No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC310100.5255914386730892No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG283560.4806085403100328No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA280410.4752695753573717RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA274860.46586282758363534No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA212520.3602021688062074RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC205380.34810051491350874RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA171570.29079562442161216No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG169690.28760919454510325No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC160780.2725075508218616RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA158240.26820248066955704No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG143520.24325341270029593RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA141790.24032121925010425RNA PCR Primer, Index 1 (100% over 23bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC141210.23933817173501107RNA PCR Primer, Index 1 (97% over 36bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC137860.2336602248805936RNA PCR Primer, Index 1 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT132570.2246941535791404No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC130980.2219992474601781No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG123800.20982979718712816Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA123070.20859251324571781No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA114310.19374510594879338No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG113750.19279595662387586No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG109210.18510106745400862Illumina Small RNA Adapter 2 (100% over 21bp)
CGTACGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAA107920.18291463418768072No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT101000.17118586038691394No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA99740.16905027440584947RNA PCR Primer, Index 1 (100% over 22bp)
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT99110.16798248141531724RNA PCR Primer, Index 1 (100% over 25bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT96840.1641350368303836RNA PCR Primer, Index 1 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCGGGGTTGG86670.14689780712607753RNA PCR Primer, Index 40 (97% over 42bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA82420.13969444171375692RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA81410.13798258310988776No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC80720.13681309554882864RNA PCR Primer, Index 1 (100% over 27bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC79170.1341859858102176RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG77690.13167751973722125No Hit
GGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAAGGA76370.12944023918562989RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCGGGGATGG70320.11918603665750285RNA PCR Primer, Index 40 (97% over 42bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTGGAAT67970.11520299931186673No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC66430.1125928386683435No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC61230.1037793092226806No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCGGGGGTGG60080.10183016328758208RNA PCR Primer, Index 40 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)