FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10111289
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA5305545.247145047481087No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC5145305.088668714740524No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2885482.8537212218936676No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG2690602.6609861512216693Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA1893091.8722538738631642No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA1478691.462414930480179No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG1421501.4058543871112774No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1313441.2989837398575No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1150711.1380448130797172No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT1074851.0630197593996176No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA1048861.037315816015149RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA937140.9268254522247362No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA922130.9119806584501738No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG870550.8609683691169345No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA838840.8296073824019865RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA827860.8187482327920803No Hit
CGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG738880.7307475832210908RNA PCR Primer, Index 7 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA720740.7128072395121927No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA716200.708317208617022RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA713750.7058941743233726No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA641290.6342316988467049No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA546040.5400300594711515RNA PCR Primer, Index 1 (100% over 28bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC544760.5387641476769184No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA530660.5248193380685687No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA530430.5245918695430425No Hit
CGGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGA494570.4891265594327291RNA PCR Primer, Index 7 (97% over 37bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG485060.47972123039901243No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG469230.46406546188126957No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC467880.4627303205357892No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG464680.45956554105020636No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG463180.4580820506663394No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC440920.4360670533697534No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC426530.4218354356205227RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA403850.399405061016454No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA393400.38907007800884735RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA380840.37664831852793446No Hit
CGCGTGGGTTCGAATCCCATCCTCGTCGCCTGGAATTCTCGGGTGCCAAG372100.3680045145579362No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA361750.357768430909254RNA PCR Primer, Index 1 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC353510.34961912373387805No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA353020.3491345168751482No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC351180.347314768670938No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC339710.3359710122023018No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT323700.3201372248384948No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC308560.3051638618973308No Hit
AAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTC291160.2879553734444738No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC278740.2756720730660552RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC275920.27288311114438524RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG265110.2621920904446505Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC264280.2613712257655775No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA255710.25289555070575076No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC249280.24653632192690764No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG245580.24287704564670243No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGTGGAATTCTCGGGTGCCAA236880.23427280142027393No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGT221160.21872582219734793No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA216420.21403799258432826RNA PCR Primer, Index 1 (100% over 28bp)
GGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTC216300.2139193133536189No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG204020.20177447207769456Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA200400.19819431528462889RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC188790.18671209971349845RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAA188630.1865538607392193No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA188020.18595057464978007No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA185490.18344842086899107No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT185450.1834088611254213No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA179710.17773203792315698No Hit
CTAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAAT173350.17144203869556096No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG160830.1590598389582179Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA157100.15537089287033531No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA154010.15231490267956935RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA150850.14918968293755622RNA PCR Primer, Index 1 (100% over 22bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG149920.1482699188995587Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA146130.14452163319632144No Hit
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA144480.14288979377406777RNA PCR Primer, Index 1 (100% over 32bp)
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG142270.1407041179418371No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGTGGAATTCTCGGGT139940.13839976287889705No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG138210.1366888039695038No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC135930.13443389858602597RNA PCR Primer, Index 1 (100% over 26bp)
GTTGAACATGGGTCAGTCGGTCCTGAGAGATGGAATTCTCGGGTGCCAAG135830.13433499922710151No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGC135020.13353391441981333No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC133350.13188229512577476No Hit
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT132510.13105154051080925RNA PCR Primer, Index 1 (100% over 30bp)
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGG132010.13055704371618693No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC128060.12665051903867053No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT120340.11901548852970181No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGGGGCTGGAATTCTCGG119670.11835286282490787No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC117520.11622652660803189RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG116070.11479248590362712No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCTGGAATTCTCGGGT115200.11393206148098427No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCTGGAATTCTCGGGT114930.11366503321188823No Hit
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA113070.11182550513589315RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT111120.10989696763686607No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC110120.10890797404762141RNA PCR Primer, Index 1 (100% over 26bp)
TAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATT108880.10768162199695805No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA106870.1056937448825763RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTTGGAATTCT104460.10331027033249668No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC104050.10290478296090637No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC102720.10158942148721098No Hit
ACAGAGTGGTTCATTTCCACCTTTCGGGCGCTGGAATTCTCGGGTGCCAA102410.10128283347454513No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)