FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9886826
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC102193310.336310156565919No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA8149138.242412681279108No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA6430036.503634230034998No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4620954.673845782256105No Hit
CTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1548451.566175029276332RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1494711.5118198701989902No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA1346201.361609883697761No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1300661.31554858960803No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1012031.0236146565136273RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA825760.8352124331914004No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC816200.8255430003521858No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC763330.7720678001210904No Hit
AAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTC759180.7678702952797996No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG709360.7174800082453155Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT693820.7017621226468435No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA648890.6563178111964345RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG588680.5954185903544778No Hit
CTAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAAT583400.5900781504600162No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC578950.5855772115338127No Hit
GCGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCCAAGGA560100.566511436531805RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG534210.5403250750038485No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA468740.4741056432064244No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG439830.4448647118903478No Hit
CGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCCAAGGAA429720.4346389832287936RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA421810.42663843785659833RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA401000.40559022683316165No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC380740.3850983116320647No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG379800.3841475514993386No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA370140.37437697396515324RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG362110.3662550549589929No Hit
CTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA361620.36575944595363563RNA PCR Primer, Index 47 (100% over 40bp)
CTGGCGGAGCGCTGAGAAGACGGTCGAACTGGAATTCTCGGGTGCCAAGG358720.36282624979948064Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT343700.347634316614857No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC343540.3474724851029036No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA338720.34259731080530803No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC333540.33735801560581724No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA318640.3222874560551587No Hit
CGGCTCGAAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA314040.3176348000864989RNA PCR Primer, Index 47 (100% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC311800.3153691589191516No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA297280.30068294920938227No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTAATCCTGGAATTCT290760.294088315097282No Hit
CTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAGGAACT288660.29196427650289386RNA PCR Primer, Index 1 (100% over 25bp)
CTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT285280.28854558581287865RNA PCR Primer, Index 1 (100% over 30bp)
CGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG279430.2826286211570832RNA PCR Primer, Index 10 (97% over 36bp)
TAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATT262130.2651305889271238No Hit
CTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGAT261230.26422028667238606RNA PCR Primer, Index 47 (100% over 41bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC258230.26118594582326016No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA249370.25222452584884164No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC248730.25157719980102816No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC245480.24828999721447512No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA242500.24527588530434338No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA241280.24404192002569886No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG237330.24004670457434973No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTTGGAATTCT232930.23559633799563176No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGT225780.22836449230521505No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC223760.22632136946680362No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTGGAATTCTCGGGTGCC222920.22547175402904837No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA221270.2238028665620291No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA219170.221678827967641No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC215680.21814887811315783No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC215600.21806796235718115No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA215180.21764315463830355RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG196820.19907298864165307Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC196660.19891115712969967No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC196310.19855715069730162No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA192070.19426861563053704No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC184180.18628829919733594No Hit
GGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAAGGAA182590.1846800985472992RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC172820.17479826184864586No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG166420.16832500137051062No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG165480.1673742412377845Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGCGGAGCGCTGAGAAGACGGTCGAATGGAATTCTCGGGTGCCAAGGA160800.1626406695131481RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA159890.16172025278891325No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG158300.16011205213887653No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTAATCTGGAATTCTC158080.15988953380994061No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC153060.15481207012240328No Hit
AAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTGGAATTCT151500.15323421288085784No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACTGGAATTCTCG149570.15128212026792018No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC148760.15046284823865616No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGC143940.14558767394106054No Hit
CTAAAGTTGGTATACAACCCCCCACTGCTAAATTTGACTGGCTTGGAATT142790.14442450994889564No Hit
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA142030.14365581026711707RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA140950.14256344756143174RNA PCR Primer, Index 47 (100% over 40bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC137470.1390436121764457RNA PCR Primer, Index 1 (100% over 24bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA136280.13783999030629243RNA PCR Primer, Index 1 (100% over 23bp)
CTGGACGCGAGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA135220.13676785653960127RNA PCR Primer, Index 1 (100% over 32bp)
CTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGGAATTCTCG133420.13494725203012573No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC132970.13449210090275687RNA PCR Primer, Index 1 (100% over 24bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG131250.132752412149258Illumina Small RNA Adapter 2 (100% over 21bp)
CTATTTTGTTGGTTTTCGGAACTGAGGCCTTGGAATTCTCGGGTGCCAAG130620.13211520057094156No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA128870.13034516840895147RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA125600.12703773688340422RNA PCR Primer, Index 1 (100% over 22bp)
CTGGACGCGAGCCGGGCCCTTCCCGTGGATCTGGAATTCTCGGGTGCCAA123810.1252272468434258No Hit
GCGGCGACTCTGGACGCGAGCCGGGCCTGGAATTCTCGGGTGCCAAGGAA120860.12224347834178532RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC117750.11909787832819146No Hit
GGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTC113260.11455648152399972No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA112420.11370686608624446RNA PCR Primer, Index 1 (100% over 22bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA110290.11155248408336507RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC107360.10858894452071878RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG106810.10803264869837903No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTTGGAATTCTCGGGTGCC106780.10800230528988779No Hit
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG106320.10753703969302181RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC105600.10680879788923159No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTATGGAATTCTCGGG101090.10224717214604566No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCTGGAATTCTCGGGTGC99040.10017370589914296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)