FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13536723
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC768700.5678626946861511No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG756030.5585029700319641No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG704060.5201111081315618No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT568330.4198431186041112No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC481610.3557803465432513No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA360940.266637649304045No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC317280.23438464390532332No Hit
GTGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCG310900.22967153867298606No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT302820.2237025903536624No Hit
GCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCT246930.18241490204091493No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG238900.1764828902829732No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC223410.16503994356684407No Hit
CCAGCAGCTCCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCC212650.1570911955574477No Hit
CCCTTCAGCCTTATGACCTCCATGCAGTGATTGTCCTTGTTTCTCACATTCTTTTCCCACAGGAGATTCTGAATA205780.1520161120235673No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC196290.14500555267327256No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA184660.13641410849583022No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG180370.1332449515292586No Hit
GTTCTTGCGAAAAATCCACAGTGGCCACAGCTAACATCATTGCAGCACCT166650.12310955908605059No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTC150580.11123814825789077No Hit
CCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA148680.10983455892537655No Hit
GCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGA144000.10637729678002572No Hit
CCACATGCTTGCCATCCAACCACTCAGTCTTGGCAGTGCAGATGAAAAAC138440.1022699511543525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)