FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15884728
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT2081701.3105040262571697No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT546190.3438459884235978No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA359830.22652575480045992No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA353110.2222952763182347No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG335400.21114620281820376No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA306050.19266933623288987No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT226800.14277864877510021No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG222800.14026050682139474No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT221660.1395428363645887No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT218570.1375975717053512No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC216970.136590314923869No Hit
ATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG215910.13592300730613707No Hit
CGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGC207700.13075452094615658No Hit
GTTTTCTCAAACTTCACTAGTGCTGTGTTATTAACATTAATTATTGTCTTATTCTATTCTCTTGGATGAATATTA205270.12922474970928052No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG204750.12889739125529878No Hit
GTTAAAAGGAGCAGAAATAGCGATATAAAAGCATTCTTCACTGGCATGTG203130.12787754376404808No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG199740.12574341845828269No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA199620.12566787419967154No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC194210.12226208720728489No Hit
CACTACTAAGTATTTTTATATGCTATATGGTCACTCTCTGTTATGAATTT193040.12152553068582603No Hit
AAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGG192810.12138073752348798No Hit
GAAATTGCGCCCCCAGCTGGCAGAGAAGAAACAGCAGTTCAGAAACCTCA192140.12095894874624229No Hit
TTAAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAAG185860.11700546587892471No Hit
AGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA185330.11667181207005872No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC180040.11334156933628324No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC175560.11052125034813312No Hit
CCAAGAGGCGGAGGGCCGCTGACGTGGAACCATCATCACCCGAACCCAAG173970.10952028892153519No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA172740.10874596027077078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)