FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14403247
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT1821541.2646731670990574No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA420190.29173282940992407No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT362380.2515960463637123No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT336230.2334404179835283No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA314000.2180063981406415No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC312940.21727045297494377No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT305840.21234100894055347No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG296010.2055161589605455No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC273570.1899363386603035No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC270820.18802704695684244No Hit
GTTATGTCACGCATCTGATGAAGCGAATTCAGAGAGGCCCAGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGG237530.16491420302658144No Hit
GTAGCAGATGCAACTTCAGAAGTTGAAGTGTCTAGCACATCTGAAACAACACCAAAAGCAGTGGTTTCTCCTCCA224900.15614534694850404No Hit
AGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA213210.14802912148906422No Hit
CCAATACAGACTTACTGCTGGCCTATGCTAATCTTATGCTTCTTACGCTTTCCACTAGAGATGTGCAGGAAAGT208790.14496036900568324No Hit
CCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTA208350.14465488233312945No Hit
CCTAAGGATACATCTTTTCTTGGTAGCGATGATATTGGAAACATTGATGTACGAGAACCAGAGCTTGAAGATTTG200000.1388575784335296No Hit
CGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAA193460.13431693561875319No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC186110.12921391961132098No Hit
GGACCCGCCAACATGGGCCGCGTTCGCACCAAAACCGTGAAGAAGGCGGCCCGGGTCATCATAGAAAAGTACTAC184670.12821414504659956No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA182880.12697136971961948No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC181730.12617293864362666No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA176640.12263901327249334No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGT175490.12184058219650055No Hit
CGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGC174390.12107686551511615No Hit
GATAGATCGCCATCATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCAACCGACTACTTCAGAGGA169760.11786231257437993No Hit
AGAAAAGTACTACACGCGCCTGGGCAACGACTTCCACACGAACAAGCGCG169100.11740408256554928No Hit
CGCCAACATGGGCCGCGTTCGCACCAAAACCGTGAAGAAGGCGGCCCGGG167690.1164251366375929No Hit
AGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAGGTTATC162400.11275235368802604No Hit
GAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTT162380.11273846793018269No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT158350.10994048772474706No Hit
AGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGGAGAG156260.10848942603011667No Hit
GAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGG154600.10733690812911839No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA152260.1057122744614461No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCA149650.10390018306288852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)