FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16607603
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG873110.5257290892611053No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC732820.4412557308842221No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG708070.4263529179978591No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT617030.3715346519302033No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC337340.20312383430649206No Hit
GTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAA276640.16657430936902815No Hit
CTTCTCCTGTTACAAGTTCTACTTTTTCACGCATTTGAACTACTGGTTCA269750.1624256071150063No Hit
GTCTAACAGAAGCCGCTGGCATTCCTCAAATTTCTGCTTGAATTCTTCAG246860.14864276319707306No Hit
CCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGG241220.14524672826054427No Hit
GGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGG232430.13995397168393298No Hit
ACTGCTTTTGGTGTTGTTTCAGATGTGCTAGACACTTCAACTTCTGAAGTTGCATCTGCTACATCATCACCCTGA222900.1342156360553657No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT210370.1266708988648151No Hit
CGAATAACATCTATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCAT200280.12059536827801098No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC197770.11908401230448487No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGGTCCTTTTTAATTCTGTCCACGTCA179700.10820345356280495No Hit
CCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTATGT178800.10766153309420992No Hit
GGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAACAGCC177000.10657769215701988No Hit
GTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAG176640.10636092396958188No Hit
CTTCCACTTCTTTGTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAA174100.10483150398043595No Hit
ACCTAAACCAAATAACACTAATAGAGAAGAACTAATTATAGATGTAAAGT171640.10335025469960957No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA167810.10104408203881077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)