FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002798768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16607603
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT2023941.2186827924535526No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC533700.3213588378768447No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC328770.1979635471777595No Hit
AGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGA318690.1918940379294953No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG304890.18358459074437172No Hit
CCGGAAGAAGGGCCGTGGTGTGATTTTTGGCCAAACAAGTAGCACTTTTA268610.16173917452145262No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA266600.16052888547492375No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA256350.1543570134714805No Hit
CACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC251830.15163536845142553No Hit
GGGAACTTAAAAATTCTCAAAAACGAGGTCAATGGCAAACTAAGAATGCTGATGCGAAGAGAACAAGTACTAAA240670.14491555464084732No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT234530.14121845277732134No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG232270.13985763026729384No Hit
ATTTTTGATGAAGCAAAAACAGCCCAGGAAAAAGATTCTTTGATAACACCTCATGTTTCTCGGTCAAGCACTCCC230210.13861723452806526No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT205930.12399742455307969No Hit
CGGCAAGTTCTCCCAGGAGAAAGCCATGTTCAGTTCGAGCGCCAAGATCG196030.11803629939853451No Hit
CTACAGAAGTGCCTTTTATGTATTGGAGCAACAGCATGTTGGCTTGGCTG195820.11790985128919568No Hit
CAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGG195460.11769308310175766No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT189660.1142007067485898No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG179530.10810109080762588No Hit
ACAGAAGTGCCTTTTATGTATTGGAGCAACAGCATGTTGGCTTGGCTGTTGACATGGATGAAATTGAAAAGTACC178980.10776991718792893No Hit
CCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTA178030.10719789002663418No Hit
GGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC176580.10632479593834221No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA173810.10465688516277756No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC171970.10354895887142776No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168770.10162213053864547No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG167000.10055635361707527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)