FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13045963
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG702900.5387873628033438No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG578630.44353184199587253No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC502450.3851382991044816No Hit
CCAGTCTACACCCCTCAGCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGGAGTGAGG402770.30873152100768647No Hit
GTCTTCTTCCACTTCTTTGTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATAC386190.2960226086797885No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT380830.2919140580116623No Hit
CCCAGCAGCTCCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTC375980.2881964328735257No Hit
GAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGG363010.2782546600814367No Hit
GGTTTAGGGACCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTT354390.27164725210396506No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC344580.26412768455651764No Hit
GTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCC340860.26127622774953446No Hit
CAGGCGCAGGACCAGGTGCAGGGTCGACTCTTTCTGGATGTTGTAGTCAG329880.25285983104505205No Hit
GCTGTTGTCCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGTCCCCGAGGCGTTTTGAA286130.21932455273711873No Hit
AACCCTGCGGATGTATTTTTCACCCAAGAAATTTCGGATTTCAACAAGAG284190.21783750268186414No Hit
GTGTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGG274240.21021062224383127No Hit
GGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACA272850.20914515854444782No Hit
AGCGTCAGACAAAGGATAAACGGTGAAAATTAACAAGAAGCAATACAGAG255690.1959916642412676No Hit
CACTCTTCATACTTGTATTTCTTCTGTTGGTTGGCCAGGAAGCCGGCCAGTTGAGTTAGAAAACATTTCTCTTTG254370.1949798569871768No Hit
TGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTG243750.1868394077156282No Hit
ACAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGG243190.18641015615328665No Hit
CTGCTGTAGGGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGT238580.18287649597043928No Hit
CGCTAGTGAAGTTTGAGAAAACCTTCTAACACACACCTGGATCGTGCGAG238120.1825238964728016No Hit
CTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGC237310.18190301474870044No Hit
GGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATC228030.17478970314418338No Hit
CTTGCTTGGAACCTTGAAGGTTTATTTGCTGTTTGGGAGGAACTACCATC224070.17175428138191104No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA223690.17146300353603638No Hit
CTCTCTTATTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATT223640.17142467750368448No Hit
CCGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTG222880.17084212181193523No Hit
CTGACTTTGGTGGAAGTCCTTGGTTACTTATCATTACTGTGTTTCTGAGA202610.15530474829646534No Hit
GATTGAAAGCAGTAATTCCATAATGGCTAGGGTTCTCTCCCTTTTGCAGG202190.1549828096247092No Hit
CCATCTCCTTCACCTTCAGGCAGTACGACAGGTACTCAAACTTCACGTCC199590.15298985594240916No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTT193170.14806879338842216No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC191970.147148968611976No Hit
CAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTACCA191610.14687302117904213No Hit
CTCTGGGTGGAGCAGGCTGGCGCTTTAGTTGAACCCAGGTACCTTTCTCT186410.14288711381444205No Hit
GTCCAAATGAACCTTTATGAGCCGGCTGCCATCTAGTTTGACGCGGATTCTCTTGCCCACAATTTCGCTTGGGA184940.1417603284632955No Hit
GGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCT184880.1417143372244732No Hit
ACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGT184410.1413540725203651No Hit
CGCCAGATTCGGGCGCTCCCATCTTGCGCAAGTTGGTCACGTGGTCACCC182330.1397597095745251No Hit
TGGGCATTTTCACCTTGGGCATCTTCAGGTGCCAGTCTGGGCCATGAACA182190.1396523966839397No Hit
GTGTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTATGTTAATTGGGAAATGAAGCAATA181120.13883221959160852No Hit
GGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACATATAGTT180110.1380580337380997No Hit
GGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGT178410.13675494863813425No Hit
CTTTAAGGCCAGCTCCCTCGGGCAGGTGGCCCTCCGGGAGCTTCACATCC176670.13542120271228733No Hit
CTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTAT175390.13444005628407807No Hit
CAGGAGGAGCAATGATCTTGATCTTCATTGTGCTGGGTGCCAGGGCAGTG173470.1329683366417642No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA173240.13279203689294533No Hit
GCTGAGTTCCTGAATGTCGCCCAAGCTACGCCACGGCGTTCAGAGAGGCG172880.1325160894600115No Hit
GTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTAT170090.13037749685477415No Hit
CACACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTAT169650.13004022777007723No Hit
CCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAA169400.1298485976083176No Hit
GGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGT168940.1294959981106799No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC168830.12941168083950566No Hit
GGATGGAGAGATATATAACTAAAGGATGCTCGTAGGTCGAATGCCAGTTGATGCCCGCAAAACAAAAACAAACAA168220.1289441032448122No Hit
CTTTTAATTACATTTATTTTAATGCTGAATTTACTCCCGTGCCATAAGTT167810.12862982977952644No Hit
GCCTAAAATTGCCAAATATGATATAGGATGACATTCCTAAAATGGAGCGGTGAACCGTCAAAGCCAGGCTATCTA167560.1284381996177668No Hit
CCCAAATAACACTAATAGAGAAGAACTAATTATAGATGTAAAGTATCTGTAAATAAATAGGAATGAGAATTAAAC164560.12613863767665137No Hit
CCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGCTGTAGGGCTGGCCTAAGTC163140.12505017835785676No Hit
CTCCGTTCCGCTGTGGGGGCTCTGGACGTAACCGCTATAATGGTGCTGGG160770.12323352442437556No Hit
GCTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGAGTTGAAT159170.122007091389114No Hit
GCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCG157010.1203514067915109No Hit
CACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGATAAAATTTATTCAGACTGTAAAGT155040.11884136111684511No Hit
TGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTATTCGATGA154850.1186957221939078No Hit
CCTATCTGAATCCAGGAGTTCAATCAGCCTCATCCCCGCAAGAAATTCTTGGAAGGCAGGACTTAAAAACCGGT154560.11847343120626665No Hit
CATCATAAGTAAACAAAAGGCGTCAGACAAAGGATAAACGGTGAAAATTA154280.11825880542509588No Hit
CGATCCCAAGAGTAGTAGCTGTTCACACGGATCACATCCACATACAGAGC151290.11596690869045082No Hit
CGCCATCGCACGCTATCACCCGAGTCTCCACCTCGCTCACGGGCTGCTCT150850.1156296396057539No Hit
TATCTCTCACTAATTATAGATGTAAAGTATCTGTAAATAAATAGGAATGA150050.11501642308812313No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAA148230.1136213555105131No Hit
CACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATAC145030.11116848943998998No Hit
CGGCTAAAGAACACAGTTACAAATACTCTAATTCCAGTGCTTGGTGGAGA144570.11081588994235228No Hit
CTCCATAAGGACTGCATTGGTTGCCTCTATTTCCCGAAGCAGGCCGTTTAAGTGATCTGCACTTTTTGTGGTGGA143350.10988073475296535No Hit
GTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAG141620.10855465403358877No Hit
CCATGCCAATCTCATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAA139730.10710593001068607No Hit
CTTCCAAATCACATGTTCGCAACAGCGAACTGATCAATCTTTCCACTGCA139600.10700628232657107No Hit
CTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAAAAAGAC139000.10654636993834797No Hit
CCTCTGGTCCTTCAATGTTAACATCAGGGCCTTCAATGTCCACTTTGGGT138850.1064313918412922No Hit
CTCTTGAATAAATTCCTAAGGATGGTGGTGATGATTTTCAAATTTAATAC138550.10620143564718067No Hit
GTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTC137690.10554222789072758No Hit
AGCTGGCAAATCGTCCAAATTCTTCAAGCCGCCAGACCGTCTCCTTCTTT137600.10547324103249411No Hit
GTACTTAAACATGCAAAAGGAGGTAACGAGAGCTGCACGTCCTTCCTTGA136420.1045687466689887No Hit
CCCCAAGTTCTTCTTCTTCTGAGTCAAAGGACAGATTTCTGATAAAAACA135540.10389420849959485No Hit
CCCCGGAAGACCCTTTTGCAAGAGACCAGATTCTGGAAGACGTTGGGCAT134330.10296671851667831No Hit
TCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGG133790.10255279736727753No Hit
CCGGCACTTATTGTAGTCGCCCTTTTCAAAGTATACCGCTGCTTGATTGG133000.10194724605611713No Hit
CTCAGACCAGGAGTCCGTGGGTCTTGAGGACCTCTGTGTATTTGTCAATT132430.1015103292873052No Hit
GGTTCTTATGAGGATGGTAAGGTAGGGTAGGGTAACTCCAGGCACTTACT132310.10141834680966058No Hit
CAACATGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCTATG131440.10075147384673712No Hit
AATTAAACAGTGGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACATATAGTTATAGAATAACGA130600.10010759650322479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)