FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13603128
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1720141.2645179843930014No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1479241.0874263625248546No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1036710.7621114790656972No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG750690.5518510154429187No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG457670.33644467654792337No Hit
GGCTTATTTTGTCTGTCCCTGTCTGAATGTATTGCAGGAACTAAAAAGGA311310.22885177585625893No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT308390.22670521074270564No Hit
CTGAACCAGGAGTCTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTG297280.21853797156065868No Hit
GCTACTACCGACACTATCATCCAAAGCATCTTCCCTTAAGTCATAGTTAT265300.1950286728170168No Hit
GTTCTTGCGAAAAATCCACAGTGGCCACAGCTAACATCATTGCAGCACCT263830.19394803900985128No Hit
CCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGG251770.18508243104086058No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC233830.17189428784320784No Hit
GGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACATATAGTT226890.16679252007332432No Hit
GGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGGGGCATGATGGGTC211350.155368676969003No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT192100.1412175199704068No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC191180.14054120493462974No Hit
AGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAA189700.13945321987707532No Hit
TGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGG187110.1375492460263551No Hit
GAGTCGAATAACATCTATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTC187060.13751248977441072No Hit
GACATTCCTAAAATGGAGCGGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTAGCCCGTATCTGC185360.13626277720830093No Hit
ATGCCTCTTTGCATCATTCTCGGGTTTCCTTAACTTATTTCTGTGAGCGT177490.13047734315225146No Hit
CCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCT176910.13005097062969634No Hit
CACCTGGTCTCATCCGAACCCTGCGGATATATTTTTCACCCAAGAAATTT175590.12908060557836404No Hit
AGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT175560.1290585518271974No Hit
GTCCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAG172390.12672820545392205No Hit
GATGACATTCCTAAAATGGAGCGGTGAACCGTCAAAGCCAGGCTATCTAA170410.1252726578769236No Hit
GTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTAT165140.12139854892198323No Hit
CACAGCACTAGTGAAGTTTGAGAAAACCTTCTAAAACACACCTGGATCGT161930.11903879754715238No Hit
GTTCTTGTGGCGCTTCTCGAAGCGGTTGTACTTGCGGATGTAGTGCAGAT161210.11850950751915294No Hit
ATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTT158490.11650996741337728No Hit
GGTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGAC158110.11623061989859979No Hit
GTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAAACATGGGACTCTTTTGCCAACTTGGGAATGTTCGCG156870.11531906485037853No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT155380.11422372854243525No Hit
GGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGG155030.1139664347788244No Hit
GTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCA154120.1132974709934362No Hit
CTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAAAATCACACATTAAAACCGGGA153260.11266526345999243No Hit
ATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGT153230.1126432097088258No Hit
GCCAGCATCCACCACATCAAACCCACTGAGTGAGCTCCCTTGTTGTTGCATGGGATGGCAATGTCCACATAGCGC150100.110342268337106No Hit
GCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTC149530.10992324706493976No Hit
GTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAA148570.10921752702760719No Hit
GGTGAAAATTAACAAGAAGCAATAAAGAGTATGGTTTAGGGCCCTGGATC147160.10818100072277494No Hit
GTTGTACTTGCGGATGTAGTGCAGATAGTCTCGGCGGATGACAATGGTCC144290.10607119186116605No Hit
GACGACATGCTTCCCACTGAACTTTTTCTCCAATTCGCGTACTAGCCGGA142930.10507142180827822No Hit
CTCTCTCCTCCTCCTGCAGCTTGATGGAGATACCTCTTACTGGGCCTCTC142500.10475531804155633No Hit
CCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCCTGGATCGTGCG141440.10397608550033492No Hit
CAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATA140590.10335122921728002No Hit
GTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCG140190.10305717920172479No Hit
GACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACT139830.10279253418772505No Hit
GCCCCTTTCTTTCCACTGCCTTACCTCAGCATCAAATCTAAATAGTTTTACCCCCTGTGAATACAGAACTTTTTC139050.10221913665739234No Hit
CCCCAGCCTTGGATGGAAACATCAAAGCCACCCACTTGGACAAGATCTCC138870.10208681415039247No Hit
GTGTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGG138750.10199859914572589No Hit
GTCTCTCCTTTCTCTCTCCTCCTCCTGCAGCTTGATGGAGATACCTCTTA138010.10145460661694869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)