FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13603128
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT1360020.9997847553886136No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC562850.4137651281381753No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT477940.3513456610861855No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG352630.25922714246311584No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT347710.25561032727178634No Hit
CCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTA325010.2389229888890261No Hit
CTGAACCAGGAGTCTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTG312740.22990300466186894No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG307960.22638910697598377No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC306350.22520555566337389No Hit
GGCTTATTTTGTCTGTCCCTGTCTGAATGTATTGCAGGAACTAAAAAGGA292020.2146712138561072No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT289830.21306129002094223No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC289820.21305393877055337No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG278480.20471762082956216No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC278250.20454854207061787No Hit
AGAGAGCTGAAGAAATGAAGAGTGGACTGAAAGATTTCAAAACATTTTTGACAAATGATCAAACAAAAGTCACTG260290.19134569637218735No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA253120.18607484984335956No Hit
AGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT237220.17438636172503855No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC232950.1712473778089863No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC212520.1562287732645021No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGT207050.1522076393017841No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC206720.15196504803895106No Hit
ATTGTCTCAGCTCAGGAATCGTCGTATATTTGATAAATCAATAATTCAAG206350.15169305177456244No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG204450.15029631420067502No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA195490.1437095938522375No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA191640.14087936245251825No Hit
GCCATCTTCCAGTAATTCGCCAAAATGACGAACACAAAGGGAAAGAGGAG184740.13580699968419027No Hit
AGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA184510.135637920925246No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT183140.1346307996219693No Hit
GCACAGTTATCGTGAAGGGCCCCAGAGGAACCCTGCGGAGGGACTTCAAT175140.1287497993108644No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC172290.12665469295003326No Hit
GGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGAT171220.12586810915842297No Hit
GGGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGAC170310.12519914537303478No Hit
CCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGC159790.11746562996393181No Hit
GGAGATTATTGAAGTAGATCCTGACACTAAGGAAATGCTGAAGCTTTTGGACTTCGGCAGTCTGTCCAACCTTC157750.11596597488460007No Hit
GTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCC157650.11589246238071126No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCG155060.11398848852999105No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA154360.11347390100276936No Hit
CTCCAATTGCTGGCCGCTTCACTCCTGGAACCTTCACTAACCAGATCCAG153470.11281963971815895No Hit
CAGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGGAGA151420.11131263338843832No Hit
AGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA151340.11125382338532726No Hit
GGTAAAATGGCCAATAAAGCAAACACTTCCGGTGACTTTGAGAAAGATGA150200.1104157808409948No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC149090.10959979204782899No Hit
CATATCGGATCATCGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTA148560.1092101757772183No Hit
ATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAG148170.10892347701205193No Hit
GAAGAAGGCGGCCCGGGTCATCATAGAAAAGTACTACACGCGCCTGGGCA144500.10622556811933255No Hit
CCCTGACTGGTAAGACCATCACCCTCGAGGTGGAGCCCAGTGACACCATCGAGAATGTCAAGGCAAAGATCCA144450.10618881186738814No Hit
AGTTCTCCCAGGAGAAAGCCATGTTCAGTTCGAGCGCCAAGATCGTGAAG143270.10532136432150017No Hit
AGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAG142000.10438775552211227No Hit
GTCAGGGTCAATTTAGACACCACTCCCCTTATACCCCACCTCCCTTCACC141390.10393932924839051No Hit
CTGCGAGAATGAAGACTATTCTCAGCAATCAGACTGTCGACATTCCAGAA139200.10232940541322555No Hit
GCCTGACGCATCTGTCTGAGGAGCGGTCAGTGACGCGATGGAGCGGGCAA138520.10182952038678163No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG136240.10015343529811672No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGT136040.1000064102903391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)