FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14942754
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT2796951.8717767822450933No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC965790.6463266409926844No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT503010.3366246944840288No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC291310.1949506764281872No Hit
CCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGA279400.18698025812377023No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC259160.17343523155102467No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA246350.16486251463418322No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC234020.15661102364396817No Hit
AGAACACTGACTTTGGGTGCGCTTAACTTACAGTACTTTTTCGACCACCCAGAAAGCTTGTCATTGTTGAGGAGC231910.15519896800817307No Hit
CCAAAACCGTGAAGAAGGCGGCCCGGGTCATCATAGAAAAGTACTACACGCGCCTGGGCAACGACTTCCACACGA229570.15363299161586946No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT229180.15337199555048553No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT223350.149470438983336No Hit
ATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT218850.14645894592121372No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA217310.14542834607328742No Hit
GGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGCTGGACTGGTATTTGTGT213940.14317307238009808No Hit
CGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGC212340.14210231929134348No Hit
GTGTGATTTTTTGAGCTGTGGTCTGTACACTTTATTGTTGTCCATCGTCT207480.1388499067842514No Hit
GCAGAGATGAACATTCTAGAAATCAACGAGAAATTGCATCCCCAGCTGGC205770.13770553942064495No Hit
CCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGC200020.13385752050793315No Hit
AGTCGCGGGCAAGCATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCT197480.13215769997953522No Hit
GGGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGAC192690.12895213292007618No Hit
CCCAGGCTCAACGGCGTGCTGATGGCAGTGGAAGAGCGTGAAGTCTTACAGGACTCACTGGATAGATGTTATTCG187130.12523126593665398No Hit
CCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCA186950.1251108062141691No Hit
AAGTCTAAAGTGACCCATGTTATGATTCGCTGTCAGGAACTGAAATACGA186300.12467581277186253No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA185140.12389951678251546No Hit
CATTATTGGAAGAAAGTTTTGCCATTGTTGAAGATAATAAAAAAGAAGAA184050.12317006624080139No Hit
ATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTTC182410.12207254432482795No Hit
CCGGAGATGCCTGACGCATCTGTCTGAGGAGCGGTCAGTGACGCGATGGAGCGGGCAAGGGATCGCTTACACCTG182120.12187847032749118No Hit
CCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTA176820.11833160072099158No Hit
GGATGAGATTCAGAATATGAAGGAGGAAATGGCTCGTCACCTTCGTGAAT175220.11726084763223701No Hit
TGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCTGGGCA173230.11592909847809847No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG172850.11567479461951925No Hit
GAATAAATTTTACCATATCGGATCATCGAATAGAAGCCGTGACGTATATA170660.11420920132928641No Hit
AGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT168360.11266999376420171No Hit
AGGCGGCCCGGGTCATCATAGAAAAGTACTACACGCGCCTGGGCAACGACTTCCACACGAACAAGCGCGTGTGCG167870.11234207563077062No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA166910.11169962377751785No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC163820.10963173187486056No Hit
GTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGG162980.10906958650326438No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT162320.10862790085415314No Hit
CCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATG160120.10715561535711556No Hit
CTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGCTGGACTGGTATTTGTGTCTGAGGCTGGCCCT157870.10564986882605441No Hit
CTGAAGAAAAGGAAGTCCCTGAGGACTCACTGGAGGAATGTGCCATCACTTATTCAAATAGCCATGGCCCTTATG155180.10384966519558576No Hit
ACAGCCCCAGCCCAAATCCTATTTCAAGAGCATCTCTGTGACCAAGATCACTAAACCAGATGGGATAGTGGAGG154340.10328751982398961No Hit
CTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTC153690.10285252638168306No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATTGTTTCCTCCCTTCCTT152000.10172154343168602No Hit
GTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG151740.10154754605476339No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC150660.10082478771985405No Hit
AGACTATTCTCAGCAATCAGACTGTCGACATTCCAGAAAATGTCGACATT149600.10011541379855414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)