FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15034608
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT1453820.9669823117436783No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA432060.28737696386896155No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC366050.24347159566780857No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT318180.21163172328802987No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA311360.20709552254372046No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG272360.18115537165983975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268860.17882740940102995No Hit
AGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGATGGCGTCACAGA258330.17182356866238213No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT251220.16709447961662852No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT248350.16518555056440448No Hit
ATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGT225120.1497345324866468No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA224380.14924233475192702No Hit
AAGGATTTTCCATCCCTGTGTCTGCTGATGGATTTAAATTTGGCATTTCGGAACCAGGAAATCAAGAAAAGAAA214450.14263757325764662No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT209120.13909241930351626No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC203280.13520804799167363No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG200680.1334787045994149No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT198920.13230807214927054No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG194360.12927506989207832No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC191090.12710008801027603No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT190000.12637509404967526No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGG188580.12543060650467242No Hit
CAGCAGTGATGATGATCTCTTTCAGTCTGCTAAACCAAAACCAGCAAAGAAAACAAATCCCTTTCCTCTCCTGGA180320.11993661557388127No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC179790.11958409557469007No Hit
CTTCCTGCTTGCCTCTATGGAAGCCAGCAGCCAATGCCTGTGAGGGCTGG170360.11331190011738251No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC166670.11085756276452302No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC165710.1102190359735352No Hit
GCCGGACAAGTCCCAGGGGCAGGACCTCCAAGAACAGCTGGCTGAGGGGTGTAGACTGGCACAGCACCTTGTCCA159440.1060486578698959No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG157920.10503765711749852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)