FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13386440
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT1537321.1484158596310894No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC691140.516298582744927No Hit
ACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGA502830.37562638012795035No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC363330.271416448286475No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC347670.2597180430345932No Hit
CCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGA331760.24783288163245792No Hit
TGCCAGCCCTACAGAAGTGCCTTTTATGTATTGGAGCAACAGCGTGTTGG285200.2130514162092386No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT281250.2101006690352327No Hit
AGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCA259700.1940022888833775No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT256310.19146987548593952No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT251280.18771234174283827No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCA226840.16945506049405218No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC225370.16835693433056137No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC223730.16713181398489815No Hit
AACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGAT216700.1618802310397686No Hit
TGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAGAATTAAAAAG208710.15591150447766547No Hit
CTATTCTCTTGGATGAATATTAATGTTTCCCGGTTTTAATGTGTGATTTT204570.15281882262946683No Hit
ATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTACACGCACTGT200440.14973361102727833No Hit
AGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCT200330.14965143832116679No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG198590.14835161551540216No Hit
CAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTT198030.14793328173883422No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC191920.14336896142663771No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATTGTTTCCTCCCTTCCTT189040.14121753057571693No Hit
AATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTT185510.13858053373413695No Hit
CCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGA185300.13842365856792396No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT185010.13820702143362984No Hit
AAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGGAGAGACA182920.13664574001751026No Hit
TGGAGCAACAGCGTGTTGGCTGGGCTCTTGACATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCA182400.13625728722498287No Hit
TACAGTCTGAATAAATTTTACCATATCGGATCATCGAATAGAAGCCGTGA182140.13606306082871922No Hit
GGAGTCCTGTGGCATCCACGAAACTACCTTCAACTCCATCATGAAGTGTG182010.13596594763058736No Hit
AAAGCGTGTTGCAGCGCCGAAGCATTGGATGCTTGACAAACTAACGGGTG177970.13294796824249017No Hit
TGGCAAGTCCATCTATGGGGAGAAATTTGAAGATGAGAACTTCATCCTAA171770.12831641571620236No Hit
CTACCGCAATGTCTGGCAGGGGCTCCGTCTTGGCTTCACTGTCTCCCCTC169590.1266879020859915No Hit
TCCTACTTTACAGTCTGAATAAATTTTACCATATCGGATCATCGAATAGAAGCCGTGACGTATATAAATTAGCCT168150.1256121866605311No Hit
GAGGCCCGTTTGAAGAAGAGTGCAGATACACTTTGGGGGATCCAAAAGGA166460.12434971508481717No Hit
GGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC165190.1234009938415292No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT164980.1232441186753162No Hit
GATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGA163340.12201899832965299No Hit
ACTCATTGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGA161830.12089099118212161No Hit
AGGAGATCGCCATTATCCCCAGCAAAAAGCTCCGCAACAAGATAGCAGGT161030.12029337150131028No Hit
GCCAGAATCTGTGTTTTAGAAGGTTTTCTCAAACTTCACTAGTGCTGTGT160930.12021866904120887No Hit
CAGCGTGTTGGCATGGCTGTTGACATGGATGAAATTGAAAAGTACCAAGA160180.11965840059044826No Hit
GAGAGAAAGGAGAGACAATTATGTTCCTGAGGTCTCAGCCTTGGATCAGG158610.11848557196685601No Hit
AAAGTTGTTGCTGTAGCCCGTGCTGTTTATCAAGCAGTGCTCAGCTTGAA155280.11599798004547886No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT154810.1156468784830022No Hit
GAAAATCCTTGTCTTCCCTTAAATCTATAGCTTCCAAAAAATCCGGGTCTTCCAGGAAATCCGTGTCTTCCAGCA152040.11357762033819298No Hit
GGAGATTTTCCTTCGGGAGTTGATCTCTAATGCTTCTGATGCCTTGGACA147980.11054470045807549No Hit
ACAAAAGATGCGCAAGACTTCTTTGCAGAAGGCTGTAAAGCTTTATTGGG147200.10996202126928444No Hit
GGATCACCTGCGACAGCTGCAGAACATCATTCAGGCCACGTCCAGGGAGATCATGTGGATCAATGACTGCGAGG146730.10961091970680778No Hit
AGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG146550.10947645527862523No Hit
GAGATCACACTGACCTGGCAGCAGGATGGGGAGGGCCATACCCAGGACACGGAGCTCGTGGAGACCAGGCCTGC145080.10837832911513443No Hit
ATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGAAGTGCTGTTTACTCATTGGAGG143910.10750431033194785No Hit
CCACATGTTTTCCGGGAAACCTCCAGAGAAGCCGCTGCCGAATGGAAAAG143650.10731008393568417No Hit
GGTCACTCTCTGTTATGAATTTTTTACTCATTACAAAGTCTTTTTACCAG142590.10651823785860916No Hit
CGCCATCATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCA140950.10529311751294593No Hit
CAGCCTTGGATCAGGAGATTATTGAAGTAGATCCTGACACTAAGGAAATG140720.10512130185471268No Hit
CGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAA139700.10435933676167823No Hit
ATGTGGGTGTTCAAGGCCCAGACTGGCACCTGAAGATGCCCAAGGTGAAA139280.10404558642925228No Hit
CGTCTCCTTTGAGCTGTTTGCAGACAAGGTCCCAAAGACAGCAGAAAATTTTCGTGCTCTGAGCACTGGAGAGAA139060.10388124101702917No Hit
GACGCCACCGCCGAGGAAAACCGTGTGCTATTAGCCATGGTCAACCCCAC138120.10317903789207586No Hit
CGTCCTCGGGAGCCGACTATGACTACTCAGAAGAGTATGATAACGAACCACAAATACCTGGCTATATTGTCGATG137440.10267106116338624No Hit
GCAATTGTTGCCATTGAAAACCCTGCTGATGTCAGTGTTATATCCTCCAGGAATACTGGCCAGAGGGCTGTGCTG137260.10253659673520368No Hit
AGAAAACATCTGCTAAAGATTTAGCACCCACATCTAAAGTGCTGGCTAAA135870.101498232539794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)