FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15062863
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1381930.9174417904484693No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC1258250.8353325659272078No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC1111030.7375955022627504No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA734760.4877957132053847No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG637450.42319312072346404No Hit
CCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTATGT583380.3872968903720362No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC547190.36327091337151507No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG498030.3306343555006774No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT497590.33034224635781395No Hit
CCCGGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT417940.2774638526553684No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGCTGTAGGGCTGGCCTAAGTC376830.2501715643301011No Hit
GGGAGATTCTGGCTGTATCGATCAGTCCATGTTGCATTACCAGCCTAAAA346700.23016872688810885No Hit
ACTACCTCCAGCAGCTCCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGGTCCTTTTTA337470.2240410737321318No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAA315210.20926300664090217No Hit
CGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCG312700.2075966567577492No Hit
TTCGGCAGGGTAGAGAGTATGTTAATTGGGAAATGAAGCAATAATGATGT293660.19495629748474774No Hit
CCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGGTCCTTTTTAATTCTGTCCACGTCA291590.1935820567444582No Hit
CCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAAT290920.193137254186007No Hit
CCCTAACATTATTGCTCAGCGTTCTTGCAGGGGCTAATCTTTGAGGCAGT288760.19170326384831357No Hit
GACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATC285610.1896120279391773No Hit
GTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATAT278630.1849781147182976No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG271170.18002553697793042No Hit
CGCACGGCCGTCTGGATCTCCCTGGAGGTGATGGTCGAGCGCTTGTTGTA249260.1654798294321604No Hit
CATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGC247000.1639794506529071No Hit
GGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTT242230.16081272199050073No Hit
CGTTTATCGAAAGAATAGAGTCTTTTTATATTTAACCTAAACCAAATAACACTAATAGAGAAGAACTAATTAT239920.1592791489904675No Hit
ACCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA234060.15538878631505842No Hit
CTCCACCACCTTCTTTGATTTGACTCTATTCAGTTGCTTTTTTACAGTGG232270.15420043321113655No Hit
GCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGAAGGG229020.15204280886044041No Hit
CCTGAAGGAGTTGAATAACATCTATCCAGTGAGTCAATTTCATCCATGTC228900.15196314273056855No Hit
CGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTT222310.14758814443177237No Hit
GCCCAGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA221350.1469508153927975No Hit
CCTCCAATGAGTAAACAGCACTGCTGTAGGGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAAT221030.1467383723798059No Hit
CCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAG216680.14385047517195104No Hit
CCTTCAGTATCTCCTTCACAGCAGCAGACTCAGATAAATTCTGTGCGCCC214800.14260237247062527No Hit
ACCCGCTGTGAATACAGAACTTTTTCATCTTCTTCTCCTGTTACAAGTTC211820.1406239969121408No Hit
AGCGGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCC207720.13790207080818567No Hit
CTGTGTATTTGTCAATTTTCTTCTCCACGTTCTTCTCGGCCTGTTTCCGT206770.1372713806133668No Hit
GCACCATTATCATCATAAGTAAACAAAAGGCGTCAGACAAAGGATAAACGGTGAAAATTAACAAGAAGCAATAC204180.13555191997696586No Hit
CTCCAATATGTAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGAGTTGAATA201380.13369304361328918No Hit
GCTGGTAATATATTGCTGCACTGAGTGTGTGCAATTTTTATTCAAGGTCA200930.13339429562626973No Hit
AGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGG195140.1295504048599526No Hit
CGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCA193870.1287072716521421No Hit
CTCGGGAACAATTAGCTCCAAAGAAACACAGACCAGGATAAGGAAGATGGGAACGGAGGAGCATAGTGAAACATA193340.12835541291187472No Hit
CGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGA188030.12483018666504503No Hit
CTCCACAGTGGCCACAGCTAACATCATTGCAGCACCTTTACTCCTTCGGC186730.12396713692476656No Hit
AGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACT179750.11933322370388684No Hit
CTCAAGAACTCGGGAACAATTAGCGCCAAAGAAACACAGACCAGGATAAG179420.11911414184673921No Hit
GGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTC176730.11732829276877842No Hit
ATCATCATCTTCATCATCATCTGCAGCATTAATTACAACTGGTGGATGCG170250.1130263217556981No Hit
TACCGGTCTGGTCTGGGGGTAGTCTTGCTAATTCTTCTTTTCTTCCCCGTTTCCCGATTTCTGAGGCTATAGCCC170210.11299976637907415No Hit
GGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGAT170060.11290018371673431No Hit
GTAAATAAATAGGAATGAGAATTAAACAGTGGAAGGAATAAAACACTGTA169610.11260143572971486No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA168930.11214999432710766No Hit
GGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGT167520.11121391730111335No Hit
GTTCCTTTTTCAGAGACATAGATACCATCCAAAAATTTCCTGATATCCTTGTTTTTAACTGTTGTGGCTTGCTGA165140.10963387239198816No Hit
GAGCGATCTCGGCACAGTAAGATTTGTTGCACATCAGCAGCACTTCCAGC163670.10865796230105791No Hit
GTCTTTTTCTTCTCATACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTC161930.10750280341791597No Hit
GTCCGAACGGTAGCCAGTTCCTTTCTGTTACCCCACCATTTGTCAACCCGGAGCCTCTTTTTTTTCTTTCCAAGA161730.1073700265347962No Hit
AGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACATCTATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTC161030.10690530744387704No Hit
CCCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA160720.1066995032750414No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA158970.10553770554774349No Hit
GCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCG157180.10434935244382161No Hit
GATAAATTCTGTGCGCCCAGATTACCTCCTGGTAGAACCACCACATCATATGGTCCCTCTTTTTTTGCATCTTCA156590.1039576606386183No Hit
ATCCAAATACAAAGATGACATCCGGTGTGGTCTTGTACATTTTGGCTAGT154700.10270291909313654No Hit
CATATAGCATATAAAAATACTTAGTAGTGTGATGATTTGGTAAGAGATAA154500.10257014221001677No Hit
GGGGAAGTGAGCATACACAGACCTCATCTTGTAACGGAAGCCCAGTGTAACACCCTTGATCATGTTCTGTACATG154130.10232450497624523No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT152570.10128884528791107No Hit
ATCTCAGCTTCGGGCCAGGAGCCAAGAGACCTGCCCAGGCTCCGATTCAG152420.10118926262557126No Hit
GGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTG151040.10027310213204489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)