FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15062863
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT2635521.7496806549989867No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC949600.6304246410526338No Hit
AAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTG554870.368369545683314No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG499710.3317496813188834No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT463500.3077104266300504No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC427860.2840495860581086No Hit
CCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGC413240.2743435959020539No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT394700.2620351788368519No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC392670.2606874934731863No Hit
CATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT370460.2459426206027367No Hit
CGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAAC352050.23372050851156254No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG338580.2247779854334465No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA314740.20895098096557077No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA312690.20759001791359316No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA311950.2070987434460501No Hit
AATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGG306220.20329468574466886No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC281740.18704279525080988No Hit
ATTTTTTAGTTAAGTGTATTAAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGA277860.1844669237182865No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG276530.18358395744554007No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT273160.18134666696497206No Hit
CGTACTATTAAATTATCTCTTACCAAATCATCACACTACTAAGTATTTTTATATGCTATATGGTCACTCTCTGTT272570.18095497515976877No Hit
GGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCACCAT271730.18039731225066574No Hit
CCGTGACTAAGGCGCAGAAGAAGGACGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCTACTCCGTATACGTGT260180.17272944725049946No Hit
AGCGAATTCAGAGAGGCCCAGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGGAGAGACAATT257400.17088384857513475No Hit
ATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT251660.16707315202959755No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT240620.15974386808138666No Hit
GCAATCATTTCCCTTGATGGAAAGAATAGGCGGAAATTGGCTGAGAGGTC235040.15603939304234526No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC234150.15544853591246233No Hit
CCAGTAGTTCAAATGCCCGAAAAAGTAGAACTTGTAACAGGAGAAGAAGA229630.15244777835395568No Hit
GAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATTGGA229580.15241458413317574No Hit
AGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAA228270.1515448955487413No Hit
CCTTTCACCTCCCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCA220570.14643298554863043No Hit
CGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGC219540.14574918460056363No Hit
AAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCT215010.14274178819790104No Hit
GAGCTCTGGTCATCCTGGCTAAAGGAGCAGAGGAAATGGAGACGGTCATC212430.14102896640565607No Hit
ACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATTGGAGCAACAGCGTGTTGGCTTGGCTGTTGACATGGATG212360.14098249449656416No Hit
TATTAATGTTTCCCGGTTTTAATGTGTGATTTTTTGAGCTGTGGTCTGTACACTTTATTGTTGTCCATCGTCTGT206600.137158520262715No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT205320.13630874821074854No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG199710.13258435663923918No Hit
CTAAGCCGCTGTGCGGTTTATAGTAGTTGGGGTATTACTACTGTATGAGT199110.13218602598987989No Hit
CCTATGATTCGCCCTTTCCCGTCCACAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGCAAA197430.13107070017167385No Hit
ACCAAGACCTGCTCAATGTTAAGATGGCCCTTGACATTGAGATTGCCACCTACAGGAAGCTGCTGGAAGGCGAGG195150.1295570437041086No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA185750.12331653019747972No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGA181630.12058132640521262No Hit
GGACGATATGTTGATGGCCTTTTCCTACCTCCGAGCAAAACTGTGTTGCC177910.11811167637918502No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA177400.11777309532722963No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC174100.11558227675575354No Hit
GAAATCGAACCTGTCGATCGACCAGAGGGATTTGTGCCATAAGAAATAGTAGCGAGAAAAAGTACAACGGAGAA173110.11492503118431072No Hit
CTCTTGTTGAAATCCGAAATTTCTTGGGTGAAAAATACATCCGCAGGGTT171320.11373667808038883No Hit
TACACATTTTCTTTTTCTACCTTCGCCCTTCGATCGGGTGTTAGGGGGAA169620.11260807457387084No Hit
AGATAGATCGCCATCATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCAACCGACTACTTCAGAGG168230.1116852752361885No Hit
GTCACTCTCTGTTATGAATTTTTTACTCATTACAAAGTCTTTTTACCAGT167360.11110769579461754No Hit
CCACTCCCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTT165770.11005211957381542No Hit
CAGTTATCGTGAAGGGCCCCAGAGGAACCCTGCGGAGGGACTTCAATCAC165110.1096139558595202No Hit
GGAAATGGAGACGGTCATCCCTGTAGATGTCATGAGGCGAGCTGGGATTAAGGTCACCGTTGCAGGCCTGGCTGG162970.10819324321013873No Hit
AGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT162590.1079409671322112No Hit
CATTTCTATAGTGTGTTACTAGTAGAAGTTTCTCTGAACGTGTAGAGCAC161270.10706463970362075No Hit
ATCGCCATCATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCAACCGACTACTTCAGAGGAAACAA161100.10695177935296896No Hit
CTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGA160890.1068123636256932No Hit
CAGTAGGCAGGCAAGGTCTGCTGTGCGGAGGCTGCCGGTCGACGCTGGGC159770.10606881308022253No Hit
GTCTGATAAAAGTGACTGGCGAGCAACCAATACAGACTTACTGCTGGCCT159390.10581653700229499No Hit
CCATCATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCAAC158800.1054248451970917No Hit
TTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGA157170.10434271359966563No Hit
GAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGG157010.10423649209316982No Hit
AGGATGCCAAGGGAGGTTCTTTCTACAGAGATCACTGCCCTGTGCTAGGT154120.10231786613208924No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC154120.10231786613208924No Hit
CTTGTGAAGCCCAAGATCGTCAAAAAGAGAACCAAGAAGTTCATCCGGCACCAGTCAGACCGATATGTCAAAATT152600.10130876182037904No Hit
AATAGCGATATAAAAGCATTCTTCACTGGCATGTGTTTTAGAAGGTTTTC150840.10014032524892512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)