FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13980088
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1474941.0550291242801906No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1070590.7657963240288617No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1033640.7393658752362646No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT965790.6908325612828761No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC616900.44127046982823No Hit
AGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAA410340.29351746569835613No Hit
TGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTC376420.26925438523706No Hit
GTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTAT356890.2552845160917442No Hit
GTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCAC333600.23862510736699225No Hit
GTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTC319400.22846780363614305No Hit
CTTGGTCTTCTTCCTCTTCTTGGTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCA312920.22383263968009356No Hit
CCTGAAGGAGTCGAATAACATCTATCCAGTGAGTCCTGCAAGACTTCAGG259940.18593588252091117No Hit
CTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGT258290.18475563243950968No Hit
CGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGA257890.18446951120765476No Hit
CGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAA252520.18062833367000264No Hit
GTGGCTTATGCTTACTGGTAAAAAGACTTTGTAATGAGTAAAAAATTCATAACAGAGAGTGACCATATAGCATAT248970.17808900773729036No Hit
CTCTTCTTCGTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC243900.17446242112352942No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC241410.17268131645523263No Hit
GGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCA235160.1682106722074997No Hit
CACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATAC234250.16755974640502977No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG217890.1558573880221641No Hit
GGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGT206740.14788175868420855No Hit
CTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGA203470.14554271761379473No Hit
GGGGATGAGAATTAAACAGTGGAAGGAATAAAACACTGTAGAGAGGCTATTCGATGTCCTACATATAGTTATAGA202900.14513499485840148No Hit
CTCCTTTCTCTCTCCTCCTCCTGCAGCTTGATGGAGATACCTCTTACTGGGCCTCTCTGAATTCGCTTCATC197730.1414368779366768No Hit
CTACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTG193500.13841114590981116No Hit
CGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGA193040.138082106493178No Hit
GCACTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA190500.13626523667089935No Hit
GTCTTTTTATATTTAACCTAAACCAAATAACACTAATAGAGAAGAACTAATTATAGATGTAAAGTATCTGTAAAT190340.13615078817815737No Hit
CGTATCTGCAGGGGCGACACGCAGAGTATGGTTTAGGGCCCTGGATCGTG188370.134741641111272No Hit
GTCCTTTTTGAGTGGGTATCAACCAGAGGGAGTGAATCCAGATTAGTTTC187110.13384035923092902No Hit
ATTGCCAAATATGATATAGGATGACATTCCTAAAATGGAGCGGTGAACCG177640.1270664390667641No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC177110.12668732843455635No Hit
AACCTTCTAAAACACACCTGGATCGTGCGAGAGAAAGAAAACCATGGGAC173560.12414800250184406No Hit
GCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTC172140.12313227212875913No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT170830.12219522509443431No Hit
CCTGGATAACAACGTTGATGGGGAAGTGAGCATACACAGACCTCATCTTG167410.11974888856207486No Hit
GGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACC164240.11748137779962473No Hit
ATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATACAGACGGTGGACAACAATAAAGTGTAC160260.1146344715426684No Hit
GCTCGAACAACGGCTCCAGGTTCTGCCTCACGGTCTTGGTGCCCTGCTCCTGCAGCAGGGTCCACTTGGTGTCC160130.11454148214231556No Hit
CACCCTCCATATCCATGGGTTCCACATCGCCAGATTCAACCAAGTTTCCATTTTGAAACTGTCTTGGCCAACGGT159740.11426251394125703No Hit
CTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAAAATCACACATTAAAACCGGGA158560.11341845630728503No Hit
TACCCTCTTTCTGGGCACCGTCTGCTGCAACAGAAACCTGAAATCGTCCC156810.11216667591791983No Hit
ATCGTGATGCTGAGAAGTTTCGTTGATAACCTGTCCATCTCTAGTTTCAA155990.11158012739261727No Hit
GTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTG154820.11074322278944167No Hit
GGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTAC154640.11061446823510696No Hit
ATGCCAGTTGTCTTGCCACCACCAAAATGAGTTCTGAATCCAAATACAAAGATGACATCCGGTGTGGTCTTGTAC153640.1098991651554697No Hit
CTGGGGCATTAATATCCACTTTGGGGCCTTTAATATCCAAGTCAGGAACTTGCATGTCACCTTCCACTTTTGGC151460.10833980444186046No Hit
GTCAGAAAGAGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATGAGCC150890.10793208168646722No Hit
AGGCTGTTTCCTGTGGTTGGCAGTTTAATGAACGGTAGTAACATTGATTC150370.10756012408505583No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA147210.10529976635340206No Hit
AGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT145650.10418389354916793No Hit
GTAAAGTATCTGTAAATAAATAGGAATGAGAATTAAACAGTGGAAGGAAT145450.10404083293324047No Hit
GGCGTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACATCTA145010.10372609957820009No Hit
CTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGT144090.10306802074493378No Hit
GGCTTGAAGTTAAAGAACCTGCTGAATTCACCTGAGAACCACTGCTATTC143320.1025172373736131No Hit
ACCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA142700.10207374946423799No Hit
CGAATCCAAATACAAAGATGACATCCGGTGTGGTCTTGTACATTTTGGCT141590.10127976304584063No Hit
GCTTCAGACGCACGACCTTGAGGGCAGCAGGAACCACCATCCGCTTTTTC141200.1010007948447821No Hit
TAGTCCGAACGGTAGCCAGTTCCTTTCTGTTACCCCACCATTTGTCAACCCGGAGCCTCTTTTTTTTCTTTCCA140600.10057161299699972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)