FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13980088
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT2770271.9815826624267316No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT556800.39828075474203023No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT556500.39806616381813903No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC540420.3865640902975718No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC459820.3289106620788081No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC408910.29249458229447484No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC400930.28678646371896943No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT387480.27716563729784816No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG384360.27493389168937993No Hit
AAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTG336770.2408926181294424No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG304970.21814598019697728No Hit
GTTAAAAGGAGCAGAAATAGCGATATAAAAGCATTCTTCACTGGCATGTG290800.20801013555851725No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGT286550.20497009747005882No Hit
AATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTT281520.20137212297948337No Hit
CCCTTATACCCCACCTCCCTTCACCTCCCTATGATTCGCCCTTTCCCGTC280350.20053521837630778No Hit
CTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTT267900.1916296950348238No Hit
ATTAAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAA259670.1857427506894091No Hit
GGAGCAACAGCGTATTGGCTTGGCTGTTGACATGGATGAAATTGAAAAGT256820.1837041369124429No Hit
GTGATTTTTTGAGCTGTGGTCTGTACACTTTATTGTTGTCCATCGTCTGT251940.18021345788381304No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA244570.1749416741868864No Hit
AAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGG237930.17019206173809492No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA236620.1692550147037701No Hit
GTTAAAATTAGCTTTATATCAGAATTTCTTATTTTTTAGTTAAGTGTATT234040.16740953275830597No Hit
GTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG229210.16395461888365795No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT217070.15527083949686155No Hit
CCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGAACACTTGCACAC213150.15246685142468344No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG204080.14597905249237345No Hit
CCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCAATAAATTTT195580.13989897631545667No Hit
CTCTCTTAGACGCTTGAATCATGAACGCCCTCGTACTATTAAATTATCTCTTACCAAATCATCACACTACTAAGT195290.13969153842236187No Hit
AAGGGCCCCAAAGTCAAGGGCGATGTGGATGTTTCTCTGCCCAAAATGGA191670.13710214127407497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183420.13120089086706752No Hit
ATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAG179930.12870448311913346No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC178950.12800348610108891No Hit
AAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGT174040.12449134798006994No Hit
ATCCAGAGGGTGAGGACCGAGGAGCGCGAGCAGATCAAGACCCTCAACAA171070.12236689783354726No Hit
GCCATCTTCCAGTAATTCGCCAAAATGACGAACACAAAGGGAAAGAGGAG168350.12042127345693387No Hit
TTACTCTGAAGGGACGCACAGTTATCGTGAAGGGCCCCAGAGGAACCCTG166820.11932685974508889No Hit
GCCAGTGGGTACTGAACTTCCAGCAGGTACTCTACCCAGCGAGCAGCTGC166730.11926248246792151No Hit
GGAGGAAATGGCTCGTCACCTTCGTGAATACCAAGACCTGCTCAATGTTA163340.11683760502795117No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC161090.11522817309876734No Hit
CGAGGACATGGGGCTGGAGGAGCCTCTATTCTTACCTTCTGGGATGCTAGGCTGTATAAAATGGCAGTTGGATTT160030.11446995183435184No Hit
GATCGCCATCATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCAACCGACTACTTCAGAGGAAACA157990.1130107335518918No Hit
CGCCATCATGAACGACACCGTAACTATCCGCACTAGAAAGTTCATGACCA156560.11198785014801052No Hit
GCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAAC156170.11170888194695199No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC156090.111651657700581No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCG149150.10668745432789836No Hit
GTCGACATTACTCTGAAGGGACGCACAGTTATCGTGAAGGGCCCCAGAGGAACCCTGCGGAGGGACTTCAATCAC147030.10517101179906736No Hit
AGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCC146910.10508517542951087No Hit
GGAAGAGAACTTTGCCGTTGAAGCTGCTAACTACCAAGACACTATTGGCC145790.10428403598031716No Hit
TGGCCGTACTCTTTCTGACTACAACATCCAGAAGGAGTCGACCCTGCACC145760.10426257688792803No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA145230.10388346625572029No Hit
AATGGCTGGTAAGCAGGCCGTTTCAGCATCAGGCAAGTGGCTGGATGGTATTCGAAAATGGTATTACAATGCTGC144530.10338275409997419No Hit
TGGAAGAGAACTTTGCCGTTGAAGCTGCTAACTACCAAGACACTATTGGC143630.10273898132830066No Hit
CCGAAGATGGCGGAGGTGCAGGTCCTGGTGCTTGATGGTCGAGGCCATCT142200.10171609792441935No Hit
CAGAGACACGGCTTCAGCATATATTCGCGAACATTCCCAAGTTGGCAAAA141920.10151581306212093No Hit
CATTAAGAACCCAGTGAATAATGTGAGGCCTGGTTTTCCAGTTTCTCTTC141810.10143712972336082No Hit
CTACAGAAGTGCCTTTTACATATTGGAGCAACAGCGTGTTGGCTTGGCTA141780.1014156706309717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)