FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13654342
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1246250.9127133332386138No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG960810.7036662769981885No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC859480.6294554508741614No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT740000.5419521497264387No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC288830.21152978298038821No Hit
GGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGT258440.1892731264531092No Hit
GTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAA226040.16554441070833No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA211540.1549250780447714No Hit
GGATCAATAGGTTCAGGAATACTGTTCTTCTTTATTATCTTCAACAATGG209690.1535701976704553No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC194560.14248947331185932No Hit
CTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAAAATCACACATTAAAACCGGGA192110.14069517227560288No Hit
CAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGG191400.14017519115897345No Hit
TGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATT184400.1350486167696693No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG179810.1316870487058256No Hit
CTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGT173050.12673624258129756No Hit
CTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGT168410.12333805612895882No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA166350.12182937852296362No Hit
GTCAAGAGAAAAGCCAACATGTTTTTTCCTCCAATGCATAAAAGGAACTT165110.12092124248828688No Hit
CCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA161630.11837260264903281No Hit
GGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCC161140.11801374244178153No Hit
GAGAATTAAACAGTGGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACATATAGTTATAGAATAACG158700.11622676508322408No Hit
CTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGAAGGG152940.11200832672859666No Hit
GGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTT150840.1104703544118054No Hit
CACACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTAT145230.10636177122266309No Hit
GTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGG143150.10483844626126987No Hit
CTCCAAAATTGCTTCCATCATTACCAAATCCATTATAGCCATCCCCACTG142630.10445761502092155No Hit
GTTTTGTCAAGAAAGGGTGTAACGCAACTAAGTCATAGTCCGCCTAGAAG141960.10396692861508815No Hit
CTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCA140630.10299287948112036No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT140300.10275119811705316No Hit
CTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGCAGAGTATGGTTTAGGGCCCTGGATCGTGCG140050.10256810617457802No Hit
CTGTAAATAAATAGGAATGAGAATTAAACAGTGGAAGGAATAAAACACTG138960.10176982530538638No Hit
CCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAA138940.10175517794998837No Hit
CGAGGCGTTTTGAAATTCATCCCAACTGTAGGCTGAGTGACCTGAAGGTT137790.10091295501460266No Hit
CAGAGTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGG137030.10035635550947823No Hit
CATCCGGCTGCATAAAAATCAAGCTCAAGAACTCGGGAACAATTAGCGCCAAAGAAACACAGACCAGGATAAGGA136950.10029776608788618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)