FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9688121
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3366653.47502885234402No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC1111851.1476425614419967No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGG582910.6016749790800506No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC539830.5572081521277449No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC521760.5385564445365618No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC487890.5035961049619426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG381970.3942663391590588No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG377180.38932213996914367No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA256480.2647365779184632No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230590.23801312968737692No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT207380.2140559557420887No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG198680.2050758862322219No Hit
ACCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA186500.19250378891840844No Hit
GCTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC130160.13435009740278842No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG129580.13375142610213064No Hit
GAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGG124310.1283117748013263No Hit
GTCTCAATGAAGTCACACAAATGGGGGTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTC123720.12770278158169165No Hit
GGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCACATTTTTTTCCAAAT122950.12690799382047355No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGCTGTAGGGCTGGCCTAAGTC122390.12632996635776947No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG109520.11304565663455277No Hit
GTCACGCACGATTTCCCGCTCGGCCGTGGTGGTGAAGCTGTAGCCGCGCTCGGTGAGGATCTTCATGAGGTAGTC108280.11176573868142232No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAA107520.1109812728391811No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC104850.10822532047235991No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104420.10778147795635501No Hit
GTCACACAAATGGGGGTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCA100260.10348755966198193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)