FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5592593
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1026551.8355528464166802No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT510810.913368807635385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG273830.4896297656561098No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTC197790.3536642126469779No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG197250.35269864980340965No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG188350.33678474367793254No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG164470.29408540904013575No Hit
CCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAG152360.27243176823344734No Hit
GTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACT144130.2577158752657309No Hit
GTTGCAACTGTTAGTGGAGCCCCATGAAATGTCTGTGACCCCTGATATCC134330.2401926977343068No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT130790.23386289687091477No Hit
GTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTG122170.21844965296062133No Hit
AGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT121830.2178417059850413No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT117530.21015296482329396No Hit
GCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCT116620.20852581262394743No Hit
AGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTT113130.2022854157275525No Hit
GTATGGTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCG112320.20083707146220012No Hit
GATCTGACTCCGGGCATCTGCCAGGCAGTTGTGGGGGTTCAGTAGCAGGGATTTCATCTGATCTTTTACAGAGAA111080.19861985308067295No Hit
GTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTAT109680.19611654200475523No Hit
GCATGGGTTGATTGGCACAAAAGTAGGTCTGCTGATAAAGAATGGAAGTA104620.18706886054465255No Hit
ACCACATGCTTGCCATCCAACCACTCAGTCTTGGCAGTGCAGATGAAAAACTGGGAACCATTTGTGTTGGGTCC99550.17800329829115044No Hit
ATGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACA99090.17718078179477748No Hit
CTCTCGTTGATTTCTAGAATGTTCATCTCTGCCTTCTCGCTGGACAAGGG97940.17512449055384505No Hit
GGGTGGATGCGTGGGGCTAGGAACATTTTCGGAACTTTCTACTTTCATAA96750.172996676139315No Hit
GATCTAATCCTTGGGGGTCTTCTTAGGGCGCGACGTCGAGGCCAACCTTG92050.16459270324159114No Hit
GGTGTTGATGATGGTTTTGAGTCTTTTCCATTCTGATTTGACTTTTGTGCATTTTTGGCTGGAGTATCTCGTAT90700.16217879613267047No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT89620.16024767044553392No Hit
GTCTGGATCTCGCGGGATGTGATGGTGGAGCGCTTGTTGTAGTGCGCCAG89200.1594966771227586No Hit
ATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACA87480.15642118065805968No Hit
CCGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTC82830.14810661172733292No Hit
CGCCACCACCAAAATGAGTTCTGAATCCAAATACAAAGATGACATCCGGT82580.14765959189234762No Hit
GATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGAAGGGAGGAAACCATGGA80890.14463773780784692No Hit
TGGGAATGTTCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGG76090.13605495697612896No Hit
GGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT75680.13532184444675305No Hit
GTATCGATCAAGTACTTGGATACGCATATACCGGAGGCAGGTTCGATCGG73220.1309231692704976No Hit
GGGCGAGAGAGGAAACAGTCCACCTGATGTGGGCAGGGATCTCTTTTGCA72920.13038674546851522No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC71990.1287238316823699No Hit
GGAGGGAGTTGAGTTTTCTAGCATTTAGAGGCTCCGTTCCGCTGTGGGGG71390.12765098407840514No Hit
CTGTCAGAGTACGGCTCCTGGGACGCTTTTGCTTATTTTTTGTACGGCTT71010.12697151392922745No Hit
GGGATGAGCGTAGCTTCTCTGGAGAAGGTGCTGGTGGTGGTAGCTGGAGT70580.12620263981305274No Hit
CCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTATGT70480.1260238318790586No Hit
AGTTGGAATCCATCAGTGTTCTATTTAGTCATCTTCTCCTTCATCCTCCT70320.12573773918466802No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA69570.12439667967971207No Hit
GGCTCCGTTCCGCTGTGGGGGCTCTGGACGTAACCGCTATAATGGTGCTG69540.12434303729951385No Hit
TGGTGGACAAGGGGCCAGCCTAGAGGAGCAGACGGGGTCAGCGGATGGTG69330.12396754063812618No Hit
CCGACGTTGTAGCACAGCAGCTGACCCCTCTAAAATAATGAAGAACTCAT69010.12339535524934497No Hit
CTCACGTCGGCCTCCACCTTCGGCGCAGACACATCCAGCGAGGCCTCGATGGACCTGCCTGGGGCCGACACCCCG68730.12289469303416144No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG68490.12246555399257554No Hit
TGGAGCGGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGG66960.11972979260246544No Hit
GGCTTTTTCGAGTTGGCTTAGGCAGAATTCTCCTCTGAGCGATAAAGACG65910.11785230929552715No Hit
GCCACCCATATTTCTGGGACTATGTTTTTCATTATCACCCTTGAGTCTTG65290.11674370010476356No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT65120.11643972661697355No Hit
GACTTATCATTCTTCATCTCTACTGCTTGTTTGCTCTGTTCCTTTTCAAT63640.11379336919386052No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT63200.11300661428428638No Hit
CAGGTGTACAGACATGTTCTTGTGGCGCTTCTCGAAGCGGTTGTACTTGC62910.11248807127570341No Hit
GGATCCTGTGGACAACGACAAATGCATCGGACAGTCCGACTTTCACTGGA62670.11205893223411752No Hit
GTTGCACTTATGAGAATATTGGCCCAGCTCAACTTCTACTGGGTTATATA62460.11168343557272986No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG61810.11052118400176805No Hit
GGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAAC61730.11037813765457276No Hit
GCCATACGTTTTTCGCATGTATTTCTTACACCCATCAATCACCATTTGGT61460.10989535623278862No Hit
CGTCCATCTTCCAGCTGTTTCCCAGCAAAGATCAACCTCTGCTGATCAGG61210.10944833639780331No Hit
GTGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCG60860.10882250862882387No Hit
CTCCAAGTTCAGAATGGTGCTGTTCACATTGGTGTCAATGGTGACTGTGAGTTTGGGCCTCACAGGCTTCCCAC60420.10803575371924971No Hit
TGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT59630.10662317104069616No Hit
GTCTTCACGAAGATTTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAG58780.10510330360174609No Hit
CCCTGGTTTTACTTCCACGGTTTTGTCAGAAGCATGAACTACTCTAACAG58350.10433442948557137No Hit
GCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCT57980.10367284012979311No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG57980.10367284012979311No Hit
TGTTGCCGATGAAGGTGGCCGACATCTTCAGGCCGCGGGGCGGGATGTCG57910.10354767457599723No Hit
CACTCTTTTTCCCGTACTCCGGTTAAGCTTTAATGCTTGCTGTGGGATGT57080.10206356872384599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)