FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5592593
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA310620.5554132045725481No Hit
AGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGA245940.4397602328651486No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC221090.39532646126760873No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA201900.3610132187341364No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT198370.35470129866414385No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCC195960.35039202745488546No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG177270.31697282459138365No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT170320.30454567317879205No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC165770.2964099121820594No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC142990.2556774648181979No Hit
AGTAACTTCCTTCCTGCCCCGACCTAGACGCACTGTAAGTTGTCAGGGTC136370.24384037958778693No Hit
AATCAAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCT134140.23985296265971795No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT132050.2361158768392408No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT130490.23332647306893245No Hit
ATTCACTATCACCAAGTAAAAGTTACAAGTATTCTCCCAAAACACCACCT129000.23066223485242No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG117880.21077879259227336No Hit
AGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCA114310.20439534934868314No Hit
AATCAATACACTTGGACTGATAAGTGGAGGGTGAGGTACACTAACTGGGCTGCTGATGAGCCCAAATTGAAATC109820.196366873112347No Hit
ATCATGAACGCCCTCGTACTATTAAATTATCTCTTACCAAATCATCACAC105340.18835627766941024No Hit
AGAGAAAGGATTTGGTTATAAGGGTTCCTGCTTTCACAGAATTATTCCAG103720.18545958913870544No Hit
ATTAGGCAAGAACCTGAGTTTCCCTTCCAACTGGAAGGCAGCAGCAGAGA102720.1836715097987642No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG101760.1819549536324206No Hit
ATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAG100820.18027415905287583No Hit
AGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGG99460.17784237115055576No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG99000.17701985465418277No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC96380.1723350867835367No Hit
AAGAACATCAACATCACCTTTGAGGAAGACAAAGTCAACTCAACTCTGGT95200.17022515316240605No Hit
GAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGG94540.16904502079804484No Hit
CCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATA89780.16053376313992454No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT87900.15717217398083502No Hit
CACAGAAAAAAGTAAAACTTGCTGCTGATGAAGATGATGACGATGATGATGAAGAGGATGATGATGAAGATGATG87650.1567251541458497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86990.15554502178148846No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC86450.15457945893792022No Hit
CGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAAC86000.15377482323494665No Hit
CCAAGCTGGATGGTGCGCGGCTGGAGGGGGACCTGTCCCTGGCTGAAAAG84680.1514145585062242No Hit
AGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA84010.1502165453484636No Hit
TAGGAAATGAAACTGTTTGCACATTATGGCAAGAAGGGCGCTGTTTTCGACAGGTGTGCAGGTTTCGGCACATGG83950.15010926058806712No Hit
ATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAAT83090.14857151235571764No Hit
ACTGAATACCCACCATGCCTGTATGAGCCTACGAACAGTCAACACAAAAGCGCAAGTCGTGCCCTACACAAACTA82680.14783839982634173No Hit
AAGATAGATCGCCATCATGAACGACACCGTAACTATCCGCACTAGAAAGT80580.14408343321246514No Hit
AATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCC79160.14154436054974856No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA77120.13789667869626843No Hit
CCGGCAAAATCCGCTCCGGCCCCTAAAAAGGGCTCCAAGAAAGCCGTCACCAAAGCCCAGAAGAAAGACGGCA76230.13630528808372075No Hit
ACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAG74530.13326555320582062No Hit
AGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCG72880.13031522229491757No Hit
GAGAAACTGAACAAGAAAGAGGAAGAACTCAAGGTTGCCCAAACTGATGG71790.12836621581438162No Hit
GCCACGACCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACCACT69520.124307275712715No Hit
GGGAATTTACCTACAGAGGACTCGGGATCCCTGGGCCACTGTCATACGAC69420.1241284677787209No Hit
AGGAAATTGAAGTTGGTGGTGGTCGGAAAGCTATCATAATCTTTGTTCCCGTTCCTCAACTGAAATCTTTCCAGA68530.12253707716617318No Hit
AAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCT68290.12210793812458728No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT67710.12107085210742137No Hit
AAAGCAAAGACAAATGTGAAATTGTGGGGCATCGACCGAGACAGCTATAGAAGAATCCTCATGGGAAGCACACTG67440.12058807068563723No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGA66950.11971191180906603No Hit
AATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAAGGTA66330.11860330261830247No Hit
GTCAGCGTGTATCAGGAAACACTTCACGAAGGGGCAAAAGTGGCTTCAAT64240.11486621679782526No Hit
GGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAG63600.11372184602026288No Hit
GAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGG63050.11273840238329519No Hit
AGTGGACGGAGTGGTTCGATCATGATAAAGTATCTCAGACTAAAGGTGGG62060.11096820383675336No Hit
AGTAAACCTCAAGCATCAGGCTATGCTCCTAAATCATTTCATGTTGAAGATACCCCAGTTTGTTTCTCAAGAAAC61820.11053906479516747No Hit
AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT61590.11012780654698097No Hit
ACAGAGCACAAATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCCTTCTTCATGCCAGCTGCC61300.10960926353839802No Hit
CCTTATCAAAATATAAAAATTCTACACTCGAAGCAGTTTGGAAATATTCT61030.10912648211661388No Hit
CGAGTTCGAGTCCGGCATCTCCCAGGCTCTTCTGGAGCTGGAGATGAACTCGGACCTCAAGGCTCAGCTCAGGG60890.1088761510090221No Hit
ATACCACCGAGTAGAGCTACAAATGTCAAAAATTACCAACATTTCGGCTG60880.10885827021562271No Hit
CCTGGTGCTCCGTCTCAGAGGTGGGATGCAGATCTTCGTGAAGACCCTGA60460.10810727689284738No Hit
ACCGATTAATTCTGTGCTACTCTTCGCTTCCTCGAACACCACTGTTTGTG59250.10594370089151849No Hit
GGGAGAGTGTGTGGGCTGGACCAACGGAGGTGTTTAAGATACACAATCTC59100.1056754889905273No Hit
CCTACATTGACAAGAAATGCCCCTTCACTGGTAATGTGTCCATTCGAGGGCGGATCCTCTCTGGCGTGGTGACCA58620.10481721090735549No Hit
CGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGC58330.10429866789877253No Hit
CTCGGATCATAATGGATGAGTTAACCAGCGGATATCCTTGGGGATGATTATCAGACGATCAGGGAATTTACCTAC58160.10399469441098252No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT58160.10399469441098252No Hit
GAAGCGAGATGACGAAGGGAACGTCATCGTTTGGAAAGCGTCGCAATAAG57730.10322582029480779No Hit
ACAATTTTCCAAGAGAAAAAATGACACACTGGATCCTGAACTGTTTGTTG57010.10193840317005011No Hit
AATGTTTCCCGGTTTTAATGTGTGATTTTTTGAGCTGTGGTCTGTACACT56240.10056158207829534No Hit
GTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTC56060.10023972779710592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)