FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9281807
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA535680.5771290008508042No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA435480.4691758835321614No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA351400.3785900741094918No Hit
CCTCACTGCTGCCTCCCGTAGGAGTTTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT296530.31947442992512126No Hit
CCCCATTGTAACACGTGTGTAGCCCCGGACGTAAGGGCCGTGCTGATTTG283710.3056624642162889No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC263940.2843627323860537No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG221460.23859578204976684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206060.2220041851764425No Hit
CCCCAGTCAAACTACCCACCAAACAGTGTCCCCGCAGCCGCGGGTTAGAA180710.19469269292067806No Hit
CTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTT179150.19301198570493872No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154140.16606680143209182No Hit
CCCAGCTTGATTAGCCTTTCACCCCTACCCTCAAGTCATCGAGAAGCTTTTCAACGCTTATTCGTTCGGTCCTCC150940.16261919688698548No Hit
CCCGATTATTATCCACGCCAAACTCCTCGACTAGTGAGCTGTTACGCACT141710.15267501252719434No Hit
CTCCCAGTCAAGCGCCCTTATGCCATTACACTCTGCCGACGGTTACCAAT133180.1434849916616452No Hit
CTCCCTTTAAACCCAATAAATCCGGATAACGCTCGGATCCTCCGTATTAC126820.1366328776282463No Hit
CTCCCAGTCAAGCGCCCTTATGCCATTACACTCTACGGACGGTTACCAAT126300.13607264188966653No Hit
CTCACTGCTGCCTCCCGTAGGAGTTTGGACCGTGTCTCAGTTCCAATGTG120760.13010397652095115No Hit
CCCCTATGTATCGTCGCCTTGGTGAGCCGTTACCCCACCAACTAGCTAAT117150.12621464764350304No Hit
CCTACATTTGCTTTTCCAGACGCTCCAGAATACCTCGCGGTACGCCTTCG113290.12205597466096849No Hit
CCCCAGTCAAACTACCCACCATGCAATGTCTCCCTAACGGGATTAGACCTCAGATAACAAAAGGGTCGTATTTCA108190.11656135491720525No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT108100.11646439103937412No Hit
CCCGTCAATTCCTTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTGGA101330.10917055267363347No Hit
CCCCAGTCAAACTACCCACCATACAGTGTCCCCGCTTCTGCGGGTTAGAA101180.10900894621058163No Hit
CCACGATAGATAGAGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCG98770.1064124690375484No Hit
GCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGC98490.10611080363985159No Hit
GCCCACGATAGATAGAGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTGCCACTTTAATGGGCGAAC95770.10318033977651118No Hit
CCTACATTTGCTTTTCCACACGCTCCAGCAAGGCTCACGCCTCACCTTCA94970.1023184386402346No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA94690.1020167732425378No Hit
CTCACAGTCAAGCACCCTTATGCTATTGCACTCCGCGCACGGTTACCATT93370.10059463636768143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)