FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12061767
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC2506662.078186388445408No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1905231.5795612699200705No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC1439031.1930507362644296No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA904180.7496248269428517No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGG779940.646621676575248No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG580910.4816126857698379No Hit
ACCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA425470.3527426785810073No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAG213880.17732061977320568No Hit
GAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGG203720.16889730998783178No Hit
GCTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC174830.14494559545048416No Hit
GCCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGA169040.14014530375192955No Hit
AGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAA139350.11553033647557609No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC137310.11383904199111126No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAA134740.11170834256705506No Hit
ATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGC134160.11122748433127586No Hit
GTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGG124340.10308605695997941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)