FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10453866
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC1201721.1495460148427386No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1096671.04905687522683No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT637560.6098796368730955No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG519850.4972801449722046No Hit
GTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTAT352560.3372532228746762No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG350040.3348426314245849No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC250650.23976775673229409No Hit
GCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACC241170.23069934127718877No Hit
GGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATT237440.22713128329749013No Hit
GTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCAC236080.22583032918156784No Hit
CCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTATGT221910.21227553519434816No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA221670.21204595505624427No Hit
GTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGG189650.18141613829754466No Hit
ACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTAC182550.17462439254530335No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT180210.17238598619879No Hit
TGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAA177940.1702145407258903No Hit
GGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGAC171300.16386282357168153No Hit
AGAAGAACTAATTATAGATGTAAAGTATCTGTAAATAAATAGGAATGAGA170450.1630497272492301No Hit
CTCTGCATCTCGTCCACACATGTCTTGAGCGAAGACATCAATATTTTGCT169760.1623896843521813No Hit
GCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCG163350.15625798149698877No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC157650.15080545321702038No Hit
AGGGTAGAGAGTATGTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAAAAAGACTTTGTA155530.14877749533043566No Hit
GTCCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAG154650.14793570149072124No Hit
TAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGG154010.14732348778911075No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT152720.14608949454680212No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA151190.14462592116638956No Hit
GTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACT150640.14409980001656802No Hit
CACACATAACATAACAGATCCTAAATCAAGACACAGCACCCTTTAATTAT147460.14105786318669092No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC144420.13814984810404113No Hit
CTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTAT136600.130669361937488No Hit
GTGAAAATTAACAAGAAGCAATACAGAGTATGGTTTAGGGCCCTGGATCG133100.12732131825680565No Hit
GTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTC128540.12295929563283095No Hit
CCAGTGTAACACCCTTGATCATGTTCTGTACATGACTACAAATAGTCCGA127650.12210793595402887No Hit
CACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGA124790.11937210597495701No Hit
CTGCATGACTGAATTAAGCATCTCTTTTACAGACTCCAGGGGCACAGGCAATTTCTTGACCACTGAAAGATTTGA121880.11658844680044683No Hit
GATCCGATATGGTAAAATTTATTCAGACTGTAAAGTAGGACCATAGGGGAATTAAGGAAGGGAGGAAACCATGGA121540.11626320827146626No Hit
GTTTAGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATAT121330.11606232565062533No Hit
GGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGAAGCCGTG120100.11488572744284267No Hit
CTCTCCCTCATTCTAACGGTACGCCGAGCGGCAGTCAGAGGTATCCGTCG118950.11378565594776134No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG116800.1117290005439136No Hit
GTCCTGTACCGATGTCGCGGTCAAAGCCACGAAGCCGAATTACCGGTCTG116630.11156638127942332No Hit
GCTAAGAATGCCTGCCTCAGTTCAGCCTGGAAATCCACCACAGGTACTTGCTGCTGCTGAGAACGCCTCGGGTAC114990.10999758366904645No Hit
AGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT113920.10897403888666642No Hit
GGAGCGGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGC111750.10689825180464337No Hit
GGATGCAAGGGTGAGAGAAACATTAGCACATGTGTGCAAGTGTTCTTTAC111500.10665910582745178No Hit
CTTAACTAAAAAATAAGAAATTCTGATATAAAGCTAATTTTAACTTCATAAAAGTAACACACATAACATAAC107500.10283277019238625No Hit
ATCGCGAATATATGCTGAAGCCGTGTCTCTGTGGACGGGAAAGGGCGAAT106820.1021822931344251No Hit
GGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTT106200.10158921111098995No Hit
GGAATGAGAATTAAACAGTGGAAGGAATAAAACACTGTAGAGAAGCTATT106080.10147442104193798No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT104840.10028825699506766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)