FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002798866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10453866
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT881250.8429895696003756No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT352440.3371384328056243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG345830.33081541316867846No Hit
AGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTC297950.285014175616944No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT249880.23903118712254395No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC236110.22585902669883087No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT176250.16859791392007512No Hit
CCAAGACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAG174710.1671247747005749No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA170430.16303059557105476No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTC145270.13896294442649254No Hit
AAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTA144760.1384750866330217No Hit
CCTAGACGCACTGTAAGTTGTCAGGGTCAATTTAGACACCACTCCCCTTA143650.137413278494291No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA136410.1304876109948224No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC130210.12455679076047081No Hit
GGTAAAGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATC127080.12156268312603204No Hit
AAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCT122230.11692325116851507No Hit
GAATAAATTTTACCATATCGGATCATCGAATAGAAGCCGTGACGTATATA121660.11637799834051823No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGT119520.11433090877575817No Hit
GTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCATG117710.11259949190089101No Hit
AGGACAATAATACCCCACTTTTATTTGCTATAATTTGCAAGAAAGAGAAAATGGTGGAATTTTTATTGAAAAAGA114370.1094045016456113No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCG114280.10931840909382232No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA114250.10928971157655933No Hit
CGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGCAC113870.10892620969122811No Hit
GTGTTATTAACATTAATTATTGTCTTATTCTATTCTCTTGGATGAATATT113330.10840965438049426No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA112100.1072330561727116No Hit
GGTCACTCTCTGTTATGAATTTTTTACTCATTACAAAGTCTTTTTACCAG109810.1050424790216366No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT108660.10394240752655524No Hit
ATCCTCATTACTCTCTTAGACGCTTGAATCATGAACGCCCTCGTACTATTAAATTATCTCTTACCAAATCATCAC105240.1006708905585742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)