FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6789130
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC562000.8277938410370697No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA432990.6377694932929551No Hit
GTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAG423530.6238354546164236No Hit
GTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACAACTTACAGTG394730.5814147026202179No Hit
GTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATAT390460.5751252369596693No Hit
GTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTC382890.5639750601328889No Hit
AATAGGAATGAGAATTAAACAGTGGAAGGAATAAAACACTGTAGAGAAGCTATTCGATGTCCTACATATAGTTAT381820.5623990113608077No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG360330.5307454710691945No Hit
GGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAA300670.4428697049548322No Hit
GAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTGAGAGAAACATTAGCACATGTGTGC274050.4036599682138949No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG256760.378192787588395No Hit
CCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTA252100.37132887424456446No Hit
GGAATGAGAATTAAACAGTGGAAGGAATAAAACACTGTAGAGAAGCTATT250900.36956134291138926No Hit
GTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACT249250.36713098732827326No Hit
CTAGGTCGGGGCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGT236830.34883703802990956No Hit
GTCCTACATATAGTTATAGAATAACGAAGATTCGGCAGGGTAGAGAGTAT233260.3435786323137133No Hit
GTACCATTTGATCTTAAGATGCTATGCCTCTTTGCATCATTCTCGGGTTTCCTTAACTTATTTCTGTGAGCGTT232980.34316620833597233No Hit
CCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCT227900.33568365902553055No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG218900.3224271740267162No Hit
GCCGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCT216840.3193929119047654No Hit
TCCTGAGAAAGTTAAAGAAGTATCTTAATACATGAACTGTGTGGTCAGCC208120.30654885088369205No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC207880.306195344617057No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG202100.2976817353622629No Hit
CCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGG196070.28879989041305737No Hit
GCAGGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCG192890.28411593238014293No Hit
GGAAGGAAGTTACTAGGCTAATTTATATACGTCACGGCTTCTATTCGATGATCCGATATGGTAAAATTTATTC184910.27236184901452765No Hit
GACCATATAGCATATAAAAATACTTAGTAGTGTGATGATTTGGTAAGAGA182630.2690035394814947No Hit
GCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCAC181670.2675895144149545No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT176040.2592968465768073No Hit
GGCTTCTATTCGATGATCCGATATGGTAAAATTTATTCAGACTGTAAAGT169330.24941340053880245No Hit
CCCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAG163860.24135640354507867No Hit
GGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGT160340.23617164496776466No Hit
GGGGCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAA156000.22977907331278086No Hit
GGCTATCTAAATGGTTTTAAGCGTGGCCCGTATCTGCAGGGGCGACACGC155840.2295434024683575No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG155050.22837977767401715No Hit
GGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG154280.22724561173522972No Hit
GCCCTGGATCGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGTTCGCGAATATATGCTGA147790.21768621310830696No Hit
GTAGGCTTTATCTGGGACTGGGACACAGTTACTTTAATCAAATAACTCTCGCGTGGCAATATTACTGAGGACAGA147540.21731797741389547No Hit
ACCCTGTGTGCTCTACTGGCCTCCAAAGGCATTCAGGGGATCATCAAAGA145610.2144751978530386No Hit
GTCTCGTTGTGCAGACAGTGTGGATCTTTAGCAACTCGAACGGGACGCCG142230.20949665126459502No Hit
GGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCA141900.20901058014797183No Hit
GGCTTATGCTTACTGGTAAAAAGACTTTGTAATGAGTAAAAAATTCATAACAGAGAGTGACCATATAGCATATAA141480.20839194418136048No Hit
GTATGTTAATTGGGAAATGAAGCAATAATGATGTGGCTTATGCTTACTGGTAAAAAGACTTTGTAATGAGTAAAA139310.20519565835386863No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAACTGCGTCGGTTT139080.20485688151501003No Hit
GGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCT138400.20385528042621073No Hit
CACGTATGGAGGACCGACGGACGTCGCGAAGCCCTCTTATTTCAGTTCATAAGTGCGTAATGAAGAACGGAGCA132200.19472303520480533No Hit
ACCGGTGAACCGTCAAAGCCAGGCTATCTAAATGGTTTTAAGCGTGGCCC129000.19000961831633803No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG127940.18844829897203325No Hit
CCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTCAAAA125830.1853403897112001No Hit
ATGGCTTTCATCTAAAATAACATGGTACGTGCCACCAGTTTCACGAGCAAGTACAGTGCAAACGCGAACTTCTGC122600.18058278453940343No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA121920.17958118345060414No Hit
GTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTGAGAGA120810.17794621696741705No Hit
GTGGTGATGATTTTCAAATTTAATACACTTAACTAAAAAATAAGAAATTC120270.17715082786748818No Hit
GGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTC118520.1745731780066076No Hit
GCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTG118150.17402818917887858No Hit
GCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTGAGA113670.16742940553502436No Hit
CGTGTCTCTGTGGACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAA112520.16573552134073144No Hit
GGCTACAAGTGATGAGAAAGATTCTTTTTCAAATATAACCAGAGAAAGAA109970.16197951725773405No Hit
GACCGATAATAATACGACCCACAGTTATCAGGATCTTCCCAATAAATTTC107880.15890106685245386No Hit
GTTGCTTCCATAACCAATGTTGGGCATCAAGATCTGGCCCTTGAATCTTC107820.1588126902857951No Hit
GCCGTTAGCTGAGTTCCTGAATGTCGCCCAAGCTACGCCACGGCGTTCAGAGAGGCGTAAAACAGGATCGTGGAA107590.1584739134469365No Hit
TGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGT106990.15759014778034888No Hit
GCTGGCTGTATCGATCAGTCCATGTTGCATTACCAGCCTAAAATTGCCAAATATGATATAGGATGACATTCCTAA106950.15753123006924302No Hit
GGACGAAGAGGTTTCAAATAGGGGAAGGATCGATAGCACGGAGTATGTTG106370.15667692325820834No Hit
CAGGGCACATTCAGCCAAGGACACACACGCACACGGGCACACACACACAA105680.15566059274163258No Hit
GTCTTCTCGATGCTGATGACATCCATGAATCCAGCAGGGTATGTGACATCCACTCGAACCTTGCCATCAATTTTG105260.15504195677502125No Hit
ACGGCGTTCAGAGAGGCGTAAAACAGGATCGTGGAAAGATCCGGTCGATCGATCTAATCCTTGGGGGTCTTCTT103310.15216971835861148No Hit
CCATATAGCATATAAAAATACTTAGTAGTGTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATG102440.15088825814205944No Hit
GTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGCTT101730.14984246876993076No Hit
ACGTGCGAGAGAAAGAAAACCATGGGACTCTTTTGCCAACTTGGGAATGT100060.14738265433126188No Hit
CCGCTGAATTTGAAACAAGCTCAATGTCATTTCCTTCAAGGATTAATTCATCTTTCTGGGCTTGAGATACTGAAC99710.14686712435908578No Hit
GTCCTCTGTAGGTAAATTCCCTGATCGTCTGATAATCATCCCCAAGGATATCCGCTGGTTAACTCATCCATTATG99250.14618957068136862No Hit
GTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCAAGGGTG98410.14495229874814594No Hit
CTTCTGAATGTCCCTCACTGTCACTGTCCTGGCTCACACTGGATCCCTGG97470.14356773253715865No Hit
GTGTGATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGG97070.14297855542610025No Hit
GCTATCTCTTCATCTGTGGTTTCTTAACGCATTTCCATCTTTCAGATTTGCTTAGTAAGTTTCAACTGCGTTCG95910.14126994180403085No Hit
GTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCA95440.14057765869853722No Hit
GTCATTTCCTTCAAGGATTAATTCATCTTTCTGGGCTTGAGATACTGAAC95110.14009158758191403No Hit
GGCCACATCAATGTCATACAGCTTCTTCACAGCCTGTTTAATCTGGTGCT94620.13936984562086746No Hit
CTCCACACCAACTCCTCCAGCAATTGCTAATAAGCAGTTTTCCTTAAATT91390.1346122404490708No Hit
GCGTCTAAGAGAGTAATGAGGATGCAAGGGTGAGAGAAACATTAGCACAT91020.13406725162134178No Hit
GTTCTTTTGTTACCACCAACCTTATTGCAGATCGCGCAAACCCAAATGCG90830.133787392493589No Hit
GGGTGGGCAAGTTTGTACCATTTGATCTTAAGATGCTATGCCTCTTTGCA90130.1327563325492368No Hit
ACGGGAAAGGGCGAATCATAGGGAGGTGAAGGGAGGTGGGGTATAAGGGGAGTGGTGTCTAAATTGACCCTGACA89750.1321966142937313No Hit
AGGGTGTTTGGGATTTGGGATACAAGGGACCATTGTTCACACAGACATCC89620.1320051317326373No Hit
GTCCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGA89360.13162216661044934No Hit
AGACTGTAAAGTAGGACCATAGGGGAATTAAGGAAGGGAGGAAACCATGG88860.13088569522162632No Hit
GGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATG88840.1308562363660734No Hit
GGGCGCGACGTCGAGGCCAACCTTGGGTCGTATGACAGTGGCCCAGGGAT88780.13076785979941463No Hit
CCTCCTGCAGTAGATCAGCCTGCTCAATTGCTTTAAGGACATTTTTCTTGGATGTTTCCTGAACTTCCCCTCCCA88720.13067948323275588No Hit
CGCTGCTATTGTTGGTTCCAAAAAGGATGACACTGGCGAAGGCATTCTTC88120.12979571756616826No Hit
CCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTT87580.12900032846623943No Hit
GATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCA87180.12841115135518102No Hit
CCCGCAAGCTTCAAAGGCACTGGAATAGCGCAGTTCTAACGATATTGCTTAGGCGACAGAGGAGAGAATGAATGG86850.1279250802385578No Hit
GCTGTGGCATCCTGGTACTGCTGGTACTCGGACACCAGGTCATTCATGTT86670.12765995053858153No Hit
GCTCAGCGTTCTTGCAGGGGCTAATCTTTGAGGCAGTCATGTCCACACAGATCGCGAAAGATTAAGACGAGGG85270.1255978306498771No Hit
GGGTGTTTGGGATTTGGGATACAAGGGACCATTGTTCACACAGACATCCA85210.12550945408321834No Hit
GCCCACTGTGACGATGTCACCGATCTGGACGTCCCTGAAGCAGGGGGACA83650.12321166335009051No Hit
GTGGCTTATGCTTACTGGTAAAAAGACTTTGTAATGAGTAAAAAAAATTCATAACAGAGAGTGACCATATAGCAT83490.12297599250566714No Hit
GGCAAGGACGACGGGGGGGCTAGCACGACGAGTGCGCAACATAATCGTGT83430.12288761593900839No Hit
CGCGTATGGGCCAACCATGGTGAGATTTTTTGTAGTCTCCGTTCTTTTGT83400.12284342765567902No Hit
GGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCA82690.12179763828355032No Hit
GTCCGTGCCCAATGCCTGAGTGTCTGGAGCTGTCTGCTGACTGCTGGTGG82470.12147359087246819No Hit
GGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT81600.12019213065591614No Hit
AGATCCTAAATCAAGACACAGCACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAGCTC81300.11975024782262234No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80870.11911688242823455No Hit
CTGGTTTTGGTACCCTTCCTGTCCTGGAGTCTGTTGACTTAGCCTCCCTGATCTTCCTGATTGTTCAACATAAG80590.11870445845049366No Hit
CTTAGGCGACAGAGGAGAGAATGAATGGACAGGCTTATAAGCATTAAAAG80440.11848351703384674No Hit
GCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAA80330.11832149332830569No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT79910.11770285736169435No Hit
ATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGA79340.11686327997843611No Hit
CCAGCCTAAAATTGCCAAATATGATATAGGATGACATTCCTAAAATGGAG79100.11650977371180106No Hit
GTGGTGTCTAAATTGACCCTGACAACTTACAGTGCGTCTAGGTCGGGGCA78230.11522831349524902No Hit
AGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGC77810.11460967752863768No Hit
GCCACGGTGCATAGTCAATCGTGTAAATTATTCGGCTATCACGCCCCGCC77810.11460967752863768No Hit
GTATGTATCACTTGCAGGACGAACTATTGGGATTCAGCTGGATTACCATG77720.11447711267864955No Hit
GTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCA76990.11340186445096795No Hit
GCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATA76970.11337240559541502No Hit
CGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCT76550.11275376962880368No Hit
GTCTCAGAATGGTATCGTGCCCCGTAGCGCAACCACTCATCGGGGGTGTG76030.11198783938442775No Hit
GTCATCTATTCGTTATCAGCTTCTTAACCCCCCTACAGGTCAAGGCTTAT75940.11185527453443961No Hit
CCTTATCGCGTGGCCTGCTCCAGCGCGCTCGGCCGCATACTGGTGCTCCG75860.11173743911222793No Hit
ACACTAATAGAGAAGAACTAATTATAGATGTAAAGTATCTGTAAATAAAT75860.11173743911222793No Hit
GATGATTTGGTAAGAGATAATTTAATAGTACGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGC75790.1116343331177927No Hit
GGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA75230.11080948516231093No Hit
CACCAAAGCCACCACGACCACTGAAGTTTCCTCCACGACCGAAGTTGTCA74070.10910087154024153No Hit
AGTACGAGGGCGTTCATGATTCAAGCGTCTAAGAGAGTAATGAGGATGCA73830.10874736527360648No Hit
GGCTATCACGCCCCGCCCGTACCATATTAGCCGTTTTATTGACAATTCTATAAAGAGCGATTGCACCAGTTTTCA73700.1085558827125125No Hit
GTGAGGAAGGAATCACAACACCTAAACAGTCCACGGCATCCAAGCAGACC72340.1065526805349139No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT71690.10559526772944397No Hit
CTCTTGTACATGTTTTAGAGGTTAGATAGTTTTTGTCCTCCGTACCATAA71550.10538905574057353No Hit
GATCCTATATGATATTTTAAACCACCCAACCGCTATCACGATTGATAGAC71370.10512392604059724No Hit
GGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG71280.1049913611906091No Hit
CTGCCAAAAACAAAAGAACAAAACAAAACAAAACAAAAAAACAAAAACAAAATAAAACATACTTGCAAAAGTCTA71190.10485879634062095No Hit
CTCTCCCGATCCAGGCGTTCCTGCCGCTCCAGGCGTTCCTGCCGCTCCAG71050.10465258435175051No Hit
GCACGAGCTGCCAGCAGAAGCTTCTCCCAGGTCCTCTTGAGATTTATGAT70860.10437272522399778No Hit
GTCACGGCAAAAAAACGTATAGGCAAGCTGAAGCCATCAATGCAACGCTG69350.10214858162975225No Hit
GTCACCATACAGGTAATAAAGCTGCTGCTGAGCAACTCACAGAGAAGATAGACGATGATTGCACTGACTCGAA69340.10213385220197581No Hit
ACCCTTTAATTATACAGACGATGGACAACAATAAAGTGTACAGACCACAG68870.10144156909648216No Hit
CTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTG68830.10138265138537633No Hit
CCTCCATCCTTTCTTTCTCTGGTTATATTTGAAAAAGAATCTTTCTCATCACTTGTAGCCTGAATGGAATTTGAA67940.10007173231327136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)