FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002798892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002798892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6789130
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT1098291.6177183232608596No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT671080.9884624392226987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG402580.5929773034247392No Hit
AGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCT328290.48355238447341564No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT229420.3379225320475525No Hit
GAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTAC214160.31544542526067404No Hit
ATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCA199460.2937931664292774No Hit
ATGCTATATGGTCACTCTCTGTTATGAATTTTTTACTCATTACAAAGTCT197170.290420127468468No Hit
AGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGG195570.2880634190242343No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC190140.2800653397416164No Hit
CATCACACTACTAAGTATTTTTATATGCTATATGGTCACTCTCTGTTATG185960.273908438931056No Hit
AGTAGTTTGATTGTTCGGATAATTATGTTTCACTATGCTCCTCCGTTCCC180950.26652899561504934No Hit
GTACTATTAAATTATCTCTTACCAAATCATCACACTACTAAGTATTTTTA176760.26035736537671245No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT170410.2510041787386602No Hit
GAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCA159940.23558246785670622No Hit
TTTTATTCGGAAGATGTTAAAAGGAGCAGAAATAGCGATATAAAAGCATT149010.2194832032970351No Hit
ATATTGCGGGACTTCTTAAGCAGTTTTTTAGGGAACTGCCAGAGCCCATT147610.21742108340833066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140720.20727250767034952No Hit
AAGTCTTTTTACCAGTAAGCATAAGCCACATCATTATTGCTTCATTTCCCAATTAACATACTCTCTACCCTGCCG132960.1958424717158163No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA123050.18124560878934415No Hit
CATGGACTGATCGATACAGCCAGAATCTGTGTTTTAGAAGGTTTTCTCAAACTTCACTAGTGCTGTGTTATTAAC120610.17765162841188784No Hit
TTACAGTCTGAATAAATTTTACCATATCGGATCATCGAATAGAAGCCGTG119830.1765027330453239No Hit
AATTTATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTT117780.17348320035114956No Hit
GTATTAAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAG116140.17106757419581006No Hit
GTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAATAAATTTT111050.16357029545759177No Hit
CATGAACGCCCTCGTACTATTAAATTATCTCTTACCAAATCATCACACTA108230.15941659682462997No Hit
CTGAAAAATTGACTGAACTTTCAGGAAACCCAAGAAAACATATAACGTCT108150.15929876140241828No Hit
TATCGGATCATCGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGCCCCGACCTAGACGC107900.15893052570800678No Hit
GAAAAGGTTATATCGACTTTACACACCTGAAGTACTTATGGTACGGAGGA107360.1581351366080779No Hit
ATATGGATGACATCTTCTCCTCTGGTATCCAGGCTAAGACAACCAAACCA107000.15760487720812533No Hit
GACGCATTCTTACCCGCGTAGGGGAGGTCGCTAAGGCAATAACGAAGTGTTCAGCACGTTCGCGCACGAACTTGA104880.15448223851951576No Hit
CAGCGGTTAGAGTCCCCATAATCTCGCATGACTTTTCTTATATCTTTCGT104750.1542907559584218No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC102460.15091771699761236No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG101070.1488703265366844No Hit
AAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAAGGTAAAGAAC100690.14831060828117887No Hit
GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA97940.1442600156426523No Hit
AATATTAATGTTTCCCGGTTTTAATGTGTGATTTTTTGAGCTGTGGTCTG97050.14294909657054733No Hit
GCATATATTCGCGAACATTCCCAAGTTGGCAAAAGAGTCCCATGGTTTTCTTTCTCTCGCACGATCCAGGGCCCT93190.13726353744883366No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT92100.13565802982119948No Hit
ATTAAATTTGAAAATCATCACCACCATCCTTAGGAATTTATTCAAGAGAA88530.13039962410500314No Hit
ATTCCTTGTAACGGTTATAAGCTTTAAAATTCCTTGCCACGTTCAAATTA86800.1278514330996755No Hit
GTGTGATTTTTTGAGCTGTGGTCTGTACACTTTATTGTTGTCCATCGTCT86100.12682037315532327No Hit
CGAATAGAAGCCGTGACGTATATAAATTAGCCTAGTAACTTCCTTCCTGC83120.12243100367793812No Hit
CAAACTAAGGCGTCGGTGACTCGGATCATAATGGATGAGTTAACCAGCGG81880.12060455463365703No Hit
AGGGAATTTACCTACAGAGGACTCGGGATCCCTGGGCCACTGTCATACGA81570.12014794237258676No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC81320.11977970667817527No Hit
ATTTGATCTTCGTGAATGCTTAAGTTCGGTTAATAACCGAATATAGTCTG81150.11952930640597544No Hit
AGAACTTCTTATCTGGACTGAACATGACATTAAATGTGTTTTGCAAAGTT81150.11952930640597544No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT80020.11786488106723542No Hit
AGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGG78720.11595005545629558No Hit
GTAGTTGGCAAGGGGCGGTGTGTATGTCAGGCACTGCAAGGAAGCGTTCA78510.11564073747298992No Hit
GAATGGGTCTCTTGTTGAAATCCGAAATTTCTTGGGTGAAAAATACATCC78390.11546398433967238No Hit
CAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCT76010.11195838052887484No Hit
AAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCC75900.11179635682333378No Hit
CCAAGATCGTCAAAAAGAGAACCAAGAAGTTCATCCGGCACCAGTCAGAC75860.11173743911222793No Hit
ATTCAAGAGAAGGTAAAGAACACTTGCACACATGTGCTAATGTTTCTCTCACCCTTGCATCCTCATTACTCTCTT74990.11045597889567588No Hit
CCTACGAACAGTCAACACAAAAGCGCAAGTCGTGCCCTACACAAACTAAGGCGTCGGTGACTCGGATCATAATGG74790.11016139034014667No Hit
CATATATTCGCGAACATTCCCAAGTTGGCAAAAGAGTCCCATGGTTTTCTTTCTCTCGCACGATCCAGGGCCCTA72840.1072891519237369No Hit
AATAGTTAGTTACTTTTCTGAACCCTTGGGTCCTTTTATTCCTTATTTTTTTGCTGATTCGTCGGGGTCCTCATT72320.10652322167936097No Hit
GACGGCCTTTGATTATTACCCGCCGGTTTCTTAATAGTTAGTTACTTTTC70540.10390138353515105No Hit
AGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACAC70470.10379827754071581No Hit
CCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTAATTCCCCTATGGTCCTACTTTACAGTCTGAAT70140.10331220642409263No Hit
ACTGGGCTTCCGTTACAAGATGAGGTCTGTGTATGCTCACTTCCCCATCA69480.10234006419084624No Hit
GTCCTCCGTAGAAAACTCTGCCAGGGCAGAGGGATTCGCCTATATACTGAATACCCACCATGCCTGTATGAGCCT69180.10189818135755244No Hit
CGAGATTTTGAGAGTAGGTGAGCAGAGATCGTCAAACTGTTGAAACAATC68220.10048415629101223No Hit
GGAGAGTGTGTGGGCTGGACCAACGGAGGTGTTTAAGATACACAATCTCT68120.10033686201324765No Hit
TGTTTCTCTCACCCTTGCATCCTCATTACTCTCTTAGACGCTTGAATCAT68060.10024848544658888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)