FastQCFastQC Report
Thu 9 Feb 2023
EGAF00002799960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002799960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108002372
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTAT2509605123.23657391524697TruSeq Adapter, Index 16 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTATG9772470.90483846039974TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTA7786470.7209536101670063TruSeq Adapter, Index 16 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCGACAACATCTCGTAT7419360.6869626900416594Illumina Single End PCR Primer 1 (96% over 25bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTAT4518550.41837507050308115TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGACAACATCTCGTAT2751710.2547823671872688TruSeq Adapter, Index 15 (97% over 36bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTAT2543250.23548093925196387TruSeq Adapter, Index 16 (97% over 36bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTAT1388720.12858236113554988TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACCCGACAACATCTCGTAT1296070.12000384584145984TruSeq Adapter, Index 16 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)