FastQCFastQC Report
Thu 23 Mar 2023
EGAF00002799962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002799962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56756653
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTAT13684722.4111217410935066TruSeq Adapter, Index 27 (97% over 39bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCGACAACATCTCGTAT3248400.5723381891458611Illumina Single End PCR Primer 1 (96% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTATG727100.12810832943232223TruSeq Adapter, Index 27 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATGCCTAAATCTCGTAT693350.12216188999023604Illumina Single End PCR Primer 1 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)