FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002799964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002799964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77678904
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTAT63557608.182092785449187TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTCTGCTT3468950.4465755593050077TruSeq Adapter, Index 25 (96% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTATG2544540.32757156305912866TruSeq Adapter, Index 7 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTA1994160.25671834916723335TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAATCTGAATCTCGTAT1212290.15606425136997298TruSeq Adapter, Index 5 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1172050.1508839517097203Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACGAATCTGAATCTCGTAT1111240.14305557143288222TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATATCGTAT824010.10607899411145141TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)