FastQCFastQC Report
Fri 24 Mar 2023
EGAF00002799966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002799966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68136429
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT38640785.671089689775201TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTCTGCTT2591400.38032518551860117TruSeq Adapter, Index 25 (96% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATG1741920.25565178944144545TruSeq Adapter, Index 1 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTA1094930.16069671041903297TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACAACGTGATATCTCGTAT1073600.15756622643079812TruSeq Adapter, Index 1 (97% over 34bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT807120.11845645741135039Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)