FastQCFastQC Report
Wed 11 May 2022
EGAF00002799968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002799968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12841630
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2634332.051398459541351Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT1656861.2902256177759366TruSeq Adapter, Index 7 (97% over 39bp)
GATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAAC615970.47966652208481325Illumina Single End Adapter 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA320580.24964120598397554TruSeq Adapter, Index 7 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCGCGTAT264820.20621992690959012TruSeq Adapter, Index 7 (97% over 39bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG241270.18788113346981652Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT234330.1824768351058238Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC167130.13014702962162902No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG159000.12381605761885368No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCGCGTA143740.11193283095681779TruSeq Adapter, Index 7 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)