FastQCFastQC Report
Wed 11 May 2022
EGAF00002799969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002799969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12841630
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT2321761.8079947794789293Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1836341.4299898065899734No Hit
CAAGCAGAAGACGGCATACGAGATCAGATCGGAAGAGCGTCGTGTAGGGA665160.5179716282123064Illumina PCR Primer Index 9 (96% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC558350.4347968287514902Illumina Single End PCR Primer 1 (100% over 50bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGT265830.2070064314265401Illumina Single End PCR Primer 1 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG247510.1927403296933489Illumina Single End PCR Primer 1 (100% over 30bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT195670.15237162260554152Illumina Single End PCR Primer 1 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGGGTAGGGAAAGAGT142930.11130206990857079Illumina Single End PCR Primer 1 (96% over 31bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC140650.10952659436535704No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)