FastQCFastQC Report
Thu 23 Feb 2023
EGAF00002799972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002799972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87403362
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT17279171.9769456923178768Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGCGAAATCTCGTAT4621890.5288000248777616TruSeq Adapter, Index 9 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCTGAGCCAATCTCGTAT2921290.33423085029612476Illumina Single End PCR Primer 1 (96% over 25bp)
ACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTATGCCGTCTTCTGC881060.10080390271486354RNA PCR Primer, Index 42 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)