FastQCFastQC Report
Tue 4 Apr 2023
EGAF00002799976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002799976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49778864
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCTCGTAT14025142.817488964794375TruSeq Adapter, Index 22 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT11437712.297704101885491Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCTGAGCCAATCTCGTAT1938460.38941427028145925Illumina Single End PCR Primer 1 (96% over 25bp)
ACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTATGCCGTCTTCTGC569540.11441402117975212RNA PCR Primer, Index 42 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)