FastQCFastQC Report
Thu 12 May 2022
EGAF00002800030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56935948
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACGACCATCTCGTAT3616940.6352647364368114TruSeq Adapter, Index 23 (97% over 37bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1650910.2899591660439201Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACGACCATCTCGTA779890.13697673041291944TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACGACCATCGCGTAT644780.1132465555855854TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)