FastQCFastQC Report
Thu 12 May 2022
EGAF00002800034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206248284
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTAT10828020.5249992770848945TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT4882330.23672099982174882Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTA2816990.13658246969948123TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph