FastQCFastQC Report
Thu 14 Sep 2023
EGAF00002800108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42640910
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTAT29189656.8454566283880895TruSeq Adapter, Index 7 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT10156982.381980121906404Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCTGAGCCAATCTCGTAT1748510.4100545696609195Illumina Single End PCR Primer 1 (96% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTATG900310.21113761408938034TruSeq Adapter, Index 7 (97% over 36bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTAT825590.19361453589991395TruSeq Adapter, Index 12 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTA809880.1899302805685901TruSeq Adapter, Index 7 (97% over 37bp)
ACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTATGCCGTCTTCTGC475470.11150559404102774RNA PCR Primer, Index 42 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)