FastQCFastQC Report
Mon 4 Sep 2023
EGAF00002800109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42640910
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3601640.8446442629859447Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG1056260.24771047334590188Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1006030.23593070598165Illumina Single End PCR Primer 1 (100% over 50bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT474400.11125466130999549Illumina Single End PCR Primer 1 (100% over 31bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG435010.10201705357601422Illumina Single End PCR Primer 1 (100% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG432740.10148470095971217Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph