FastQCFastQC Report
Thu 16 Mar 2023
EGAF00002800110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72045400
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT1503082820.86299472277203TruSeq Adapter, Index 10 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTATG5354270.7431799948365891TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTA4866380.6754601959320096TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTGAAATCTCGTAT3942670.5472479852981592TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGCTGAAATCTCGTAT2505060.3477057522062477TruSeq Adapter, Index 9 (97% over 35bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT2367170.3285664317222196TruSeq Adapter, Index 9 (97% over 35bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2256860.31325525293773093Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT1813590.25172877102493707TruSeq Adapter, Index 9 (97% over 35bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT1746910.24247349587898742TruSeq Adapter, Index 9 (97% over 35bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG907970.12602747711859466Illumina Single End PCR Primer 1 (96% over 32bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT805520.11180727707806466TruSeq Adapter, Index 9 (97% over 35bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACGAGCTGAAATCTCGTAT789930.10964336376784638Illumina Single End PCR Primer 1 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)