FastQCFastQC Report
Fri 24 Mar 2023
EGAF00002800111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72045400
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG15628792.1692974152409454Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5561970.7720090387450136Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG4430000.6148900554372659Illumina Single End PCR Primer 1 (98% over 50bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT2410070.33452101036291004Illumina Single End PCR Primer 1 (100% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCCG1539200.21364306395689386Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG1377680.19122386717264392Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCG1345530.18676140322629897Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG1324140.18379244198796868Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCG1279500.17759634896884466Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGT1266600.17580581133563003Illumina Single End PCR Primer 1 (97% over 37bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGGCGCCG1235400.17147520868785515Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG1082830.15029828413750218Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGGCGCCG1023890.14211733157148132Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGTCGCCG891620.12375807476952033Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGGCGCCG803050.11146443770178249Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG745660.1034986272544812Illumina Single End PCR Primer 1 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)