FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002800120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74395649
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTAT25250583.394093651901605TruSeq Adapter, Index 25 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTATG1509850.2029486966368154TruSeq Adapter, Index 25 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACAGTACAAGATCTCGTAT928880.12485676413683817Illumina Single End PCR Primer 1 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)