FastQCFastQC Report
Thu 9 Feb 2023
EGAF00002800124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002800124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96362133
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACAAGATCTCGTAT46536264.829309870091813TruSeq Adapter, Index 14 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACAAGATCTCGTATG2517420.261245773793737TruSeq Adapter, Index 14 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACAAGATCTCGTA1405950.14590274791862484TruSeq Adapter, Index 14 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACAGTACAAGATCTCGTAT1178220.12227002073521973Illumina Single End PCR Primer 1 (96% over 25bp)
CTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATT1020190.10587042526341753No Hit
GGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGG1011370.10495512796504826No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)